Transepithelial transport of 6-O-caffeoylsophorose across Caco-2 cell monolayers

Hoang Lan Phuong, Ju Qiu, Takashi Kuwahara, Keiichi Fukui, Kayo Yoshiyama, Kazusato Matsugano, Norihiko Terahara, Toshiro Matsui

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The aim of this study was to clarify the transport behaviour and mechanism of caffeic acid analogue bearing a sugar-moiety, 6-O-caffeoylsophorose (CS), in Caco-2 cells. The absorption of CS was investigated by its transport across Caco-2 cell monolayers using a high-performance liquid chromatography-time-of- flight-mass spectrometry (LC-TOF-MS). The permeation of CS was concentration-dependent and reached the plateau at >6 mM. The apparent permeability (Papp) of CS in the apical-to-basolateral direction was 5.4 × 10-7 cm/s, while in the reversed direction the P app value was significantly reduced (1.9 × 10-7 cm/s). CS transport was competitively inhibited by phloretin, an inhibitor of monocarboxylic acid transporter (MCT). Benzoic acid, an MCT substrate, also reduced CS transport. A less significant change of CS transport was observed across Caco-2 cell monolayers pretreated with quercetin, a suppressor of tight-junction. These findings strongly indicate that CS, a caffeic acid analogue bearing sophorose moiety, can be transported across Caco-2 cell monolayers via the MCT pathway.

Original languageEnglish
Pages (from-to)101-106
Number of pages6
JournalFood Chemistry
Volume138
Issue number1
DOIs
Publication statusPublished - May 1 2013

Fingerprint

Monocarboxylic Acid Transporters
Caco-2 Cells
Monolayers
Bearings (structural)
caffeic acid
Phloretin
cells
Benzoic Acid
tight junctions
Tight Junctions
benzoic acid
Quercetin
High performance liquid chromatography
Application programs
Permeation
Sugars
quercetin
Mass spectrometry
Permeability
Mass Spectrometry

All Science Journal Classification (ASJC) codes

  • Food Science
  • Analytical Chemistry

Cite this

Phuong, H. L., Qiu, J., Kuwahara, T., Fukui, K., Yoshiyama, K., Matsugano, K., ... Matsui, T. (2013). Transepithelial transport of 6-O-caffeoylsophorose across Caco-2 cell monolayers. Food Chemistry, 138(1), 101-106. https://doi.org/10.1016/j.foodchem.2012.10.137

Transepithelial transport of 6-O-caffeoylsophorose across Caco-2 cell monolayers. / Phuong, Hoang Lan; Qiu, Ju; Kuwahara, Takashi; Fukui, Keiichi; Yoshiyama, Kayo; Matsugano, Kazusato; Terahara, Norihiko; Matsui, Toshiro.

In: Food Chemistry, Vol. 138, No. 1, 01.05.2013, p. 101-106.

Research output: Contribution to journalArticle

Phuong, HL, Qiu, J, Kuwahara, T, Fukui, K, Yoshiyama, K, Matsugano, K, Terahara, N & Matsui, T 2013, 'Transepithelial transport of 6-O-caffeoylsophorose across Caco-2 cell monolayers', Food Chemistry, vol. 138, no. 1, pp. 101-106. https://doi.org/10.1016/j.foodchem.2012.10.137
Phuong HL, Qiu J, Kuwahara T, Fukui K, Yoshiyama K, Matsugano K et al. Transepithelial transport of 6-O-caffeoylsophorose across Caco-2 cell monolayers. Food Chemistry. 2013 May 1;138(1):101-106. https://doi.org/10.1016/j.foodchem.2012.10.137
Phuong, Hoang Lan ; Qiu, Ju ; Kuwahara, Takashi ; Fukui, Keiichi ; Yoshiyama, Kayo ; Matsugano, Kazusato ; Terahara, Norihiko ; Matsui, Toshiro. / Transepithelial transport of 6-O-caffeoylsophorose across Caco-2 cell monolayers. In: Food Chemistry. 2013 ; Vol. 138, No. 1. pp. 101-106.
@article{3dc06b454c264eb283547e8625a959af,
title = "Transepithelial transport of 6-O-caffeoylsophorose across Caco-2 cell monolayers",
abstract = "The aim of this study was to clarify the transport behaviour and mechanism of caffeic acid analogue bearing a sugar-moiety, 6-O-caffeoylsophorose (CS), in Caco-2 cells. The absorption of CS was investigated by its transport across Caco-2 cell monolayers using a high-performance liquid chromatography-time-of- flight-mass spectrometry (LC-TOF-MS). The permeation of CS was concentration-dependent and reached the plateau at >6 mM. The apparent permeability (Papp) of CS in the apical-to-basolateral direction was 5.4 × 10-7 cm/s, while in the reversed direction the P app value was significantly reduced (1.9 × 10-7 cm/s). CS transport was competitively inhibited by phloretin, an inhibitor of monocarboxylic acid transporter (MCT). Benzoic acid, an MCT substrate, also reduced CS transport. A less significant change of CS transport was observed across Caco-2 cell monolayers pretreated with quercetin, a suppressor of tight-junction. These findings strongly indicate that CS, a caffeic acid analogue bearing sophorose moiety, can be transported across Caco-2 cell monolayers via the MCT pathway.",
author = "Phuong, {Hoang Lan} and Ju Qiu and Takashi Kuwahara and Keiichi Fukui and Kayo Yoshiyama and Kazusato Matsugano and Norihiko Terahara and Toshiro Matsui",
year = "2013",
month = "5",
day = "1",
doi = "10.1016/j.foodchem.2012.10.137",
language = "English",
volume = "138",
pages = "101--106",
journal = "Food Chemistry",
issn = "0308-8146",
publisher = "Elsevier Limited",
number = "1",

}

TY - JOUR

T1 - Transepithelial transport of 6-O-caffeoylsophorose across Caco-2 cell monolayers

AU - Phuong, Hoang Lan

AU - Qiu, Ju

AU - Kuwahara, Takashi

AU - Fukui, Keiichi

AU - Yoshiyama, Kayo

AU - Matsugano, Kazusato

AU - Terahara, Norihiko

AU - Matsui, Toshiro

PY - 2013/5/1

Y1 - 2013/5/1

N2 - The aim of this study was to clarify the transport behaviour and mechanism of caffeic acid analogue bearing a sugar-moiety, 6-O-caffeoylsophorose (CS), in Caco-2 cells. The absorption of CS was investigated by its transport across Caco-2 cell monolayers using a high-performance liquid chromatography-time-of- flight-mass spectrometry (LC-TOF-MS). The permeation of CS was concentration-dependent and reached the plateau at >6 mM. The apparent permeability (Papp) of CS in the apical-to-basolateral direction was 5.4 × 10-7 cm/s, while in the reversed direction the P app value was significantly reduced (1.9 × 10-7 cm/s). CS transport was competitively inhibited by phloretin, an inhibitor of monocarboxylic acid transporter (MCT). Benzoic acid, an MCT substrate, also reduced CS transport. A less significant change of CS transport was observed across Caco-2 cell monolayers pretreated with quercetin, a suppressor of tight-junction. These findings strongly indicate that CS, a caffeic acid analogue bearing sophorose moiety, can be transported across Caco-2 cell monolayers via the MCT pathway.

AB - The aim of this study was to clarify the transport behaviour and mechanism of caffeic acid analogue bearing a sugar-moiety, 6-O-caffeoylsophorose (CS), in Caco-2 cells. The absorption of CS was investigated by its transport across Caco-2 cell monolayers using a high-performance liquid chromatography-time-of- flight-mass spectrometry (LC-TOF-MS). The permeation of CS was concentration-dependent and reached the plateau at >6 mM. The apparent permeability (Papp) of CS in the apical-to-basolateral direction was 5.4 × 10-7 cm/s, while in the reversed direction the P app value was significantly reduced (1.9 × 10-7 cm/s). CS transport was competitively inhibited by phloretin, an inhibitor of monocarboxylic acid transporter (MCT). Benzoic acid, an MCT substrate, also reduced CS transport. A less significant change of CS transport was observed across Caco-2 cell monolayers pretreated with quercetin, a suppressor of tight-junction. These findings strongly indicate that CS, a caffeic acid analogue bearing sophorose moiety, can be transported across Caco-2 cell monolayers via the MCT pathway.

UR - http://www.scopus.com/inward/record.url?scp=84869862187&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84869862187&partnerID=8YFLogxK

U2 - 10.1016/j.foodchem.2012.10.137

DO - 10.1016/j.foodchem.2012.10.137

M3 - Article

VL - 138

SP - 101

EP - 106

JO - Food Chemistry

JF - Food Chemistry

SN - 0308-8146

IS - 1

ER -