Transepithelial transport of 6-O-caffeoylsophorose across Caco-2 cell monolayers

Hoang Lan Phuong, Ju Qiu, Takashi Kuwahara, Keiichi Fukui, Kayo Yoshiyama, Kazusato Matsugano, Norihiko Terahara, Toshiro Matsui

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)


The aim of this study was to clarify the transport behaviour and mechanism of caffeic acid analogue bearing a sugar-moiety, 6-O-caffeoylsophorose (CS), in Caco-2 cells. The absorption of CS was investigated by its transport across Caco-2 cell monolayers using a high-performance liquid chromatography-time-of- flight-mass spectrometry (LC-TOF-MS). The permeation of CS was concentration-dependent and reached the plateau at >6 mM. The apparent permeability (Papp) of CS in the apical-to-basolateral direction was 5.4 × 10-7 cm/s, while in the reversed direction the P app value was significantly reduced (1.9 × 10-7 cm/s). CS transport was competitively inhibited by phloretin, an inhibitor of monocarboxylic acid transporter (MCT). Benzoic acid, an MCT substrate, also reduced CS transport. A less significant change of CS transport was observed across Caco-2 cell monolayers pretreated with quercetin, a suppressor of tight-junction. These findings strongly indicate that CS, a caffeic acid analogue bearing sophorose moiety, can be transported across Caco-2 cell monolayers via the MCT pathway.

Original languageEnglish
Pages (from-to)101-106
Number of pages6
JournalFood Chemistry
Issue number1
Publication statusPublished - May 1 2013

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Food Science


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