Transepithelial transport of theasinensins through Caco-2 cell monolayers and their absorption in sprague-dawley rats after oral administration

Ju Qiu, Yuko Kitamura, Yuji Miyata, Shizuka Tamaru, Kazunari Tanaka, Takashi Tanaka, Toshiro Matsui

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

The aim of this study is to illustrate the in vivo and in vitro absorption of theasinensins B and A that are (-)-epigallocatechin-3-O-gallate (EGCG)-(-)-epigallocatechin (EGC) dimer and EGCG dimer, respectively, and their transport pathway across the intestinal membrane. Our animal study by a single oral administration to rats demonstrated the intact absorption of theasinensins into the blood system, which was estimated to be a >10-fold lower absorption amount than EGCG. The in vitro absorption study indicated that theasinensins can be transported across Caco-2 cell monolayers, while their permeability coefficients were also >10-fold lower than those of EGCG and EGC. Transport experiments using cytochalasin D or quercetin as a tight junction (TJ) modulator and a non-saturable permeation revealed that theasinensins were transported across Caco-2 cells in a TJ paracellular diffusion route. In conclusion, the dimers of condensed catechins, theasinensins B and A, can be absorbed intact into rat blood and transported across Caco-2 cell monolayers probably through a TJ paracellular pathway.

Original languageEnglish
Pages (from-to)8036-8043
Number of pages8
JournalJournal of Agricultural and Food Chemistry
Volume60
Issue number32
DOIs
Publication statusPublished - Aug 15 2012

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

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