Transepithelial transport of theasinensins through Caco-2 cell monolayers and their absorption in sprague-dawley rats after oral administration

Ju Qiu, Yuko Kitamura, Yuji Miyata, Shizuka Tamaru, Kazunari Tanaka, Takashi Tanaka, Toshiro Matsui

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The aim of this study is to illustrate the in vivo and in vitro absorption of theasinensins B and A that are (-)-epigallocatechin-3-O-gallate (EGCG)-(-)-epigallocatechin (EGC) dimer and EGCG dimer, respectively, and their transport pathway across the intestinal membrane. Our animal study by a single oral administration to rats demonstrated the intact absorption of theasinensins into the blood system, which was estimated to be a >10-fold lower absorption amount than EGCG. The in vitro absorption study indicated that theasinensins can be transported across Caco-2 cell monolayers, while their permeability coefficients were also >10-fold lower than those of EGCG and EGC. Transport experiments using cytochalasin D or quercetin as a tight junction (TJ) modulator and a non-saturable permeation revealed that theasinensins were transported across Caco-2 cells in a TJ paracellular diffusion route. In conclusion, the dimers of condensed catechins, theasinensins B and A, can be absorbed intact into rat blood and transported across Caco-2 cell monolayers probably through a TJ paracellular pathway.

Original languageEnglish
Pages (from-to)8036-8043
Number of pages8
JournalJournal of Agricultural and Food Chemistry
Volume60
Issue number32
DOIs
Publication statusPublished - Aug 15 2012

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Caco-2 Cells
epigallocatechin
oral administration
Oral Administration
Sprague Dawley Rats
Rats
Tight Junctions
Monolayers
Dimers
tight junctions
rats
Blood
Cytochalasin D
Catechin
Quercetin
Hydraulic conductivity
cells
Permeation
Modulators
Animals

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)

Cite this

Transepithelial transport of theasinensins through Caco-2 cell monolayers and their absorption in sprague-dawley rats after oral administration. / Qiu, Ju; Kitamura, Yuko; Miyata, Yuji; Tamaru, Shizuka; Tanaka, Kazunari; Tanaka, Takashi; Matsui, Toshiro.

In: Journal of Agricultural and Food Chemistry, Vol. 60, No. 32, 15.08.2012, p. 8036-8043.

Research output: Contribution to journalArticle

Qiu, Ju ; Kitamura, Yuko ; Miyata, Yuji ; Tamaru, Shizuka ; Tanaka, Kazunari ; Tanaka, Takashi ; Matsui, Toshiro. / Transepithelial transport of theasinensins through Caco-2 cell monolayers and their absorption in sprague-dawley rats after oral administration. In: Journal of Agricultural and Food Chemistry. 2012 ; Vol. 60, No. 32. pp. 8036-8043.
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