Transfer of high-mannose-type oligosaccharides to disaccharides by Endo-β-N-acetylglucosaminidase from Arthrobacter protophormiae

Kiyotaka Fujita, Tsuyoshi Miyamura, Mutsumi Sano, Ikunoshin Kato, Kaoru Takegawa

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Endo-β-N-acetylglucosaminidase from Arthrobacter protophormiae (Endo-A) has transglycosylation activity, and high-mannose-type oligosaccharides are transferred to suitable glycosides as acceptor substrates. The acceptor specificity of Endo-A-catalyzed transglycosylation toward various disaccharides was investigated. To identify an effective acceptor for the transglycosylation by Endo-A, the reaction was carried out using various disaccharides. Endo-A transferred high-mannose-type oligosaccharides more efficiently to β-linked disaccharides (cellobiose, gentiobiose, sophorose, and laminaribiose) than to α-linked disaccharides (isomaltose, maltose, nigerose, kojibiose, and trehalose) as acceptor substrates. The transglycosylation products, (Man)6GlcNAc-Glcβ-Glc, were more rapidly hydrolyzed than (Man)6GlcNAc-Glc-α-Glc. These results indicate that Endo-A recognizes the anomeric configuration of the acceptor substrates, and β-linked glycosides are suitable for the synthesis of transglycosylation products.

Original languageEnglish
Pages (from-to)614-617
Number of pages4
JournalJournal of Bioscience and Bioengineering
Volume93
Issue number6
DOIs
Publication statusPublished - Jan 1 2002
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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