Transfer of the E. coli O6-methyltransferase gene into repair-deficient human cells and restoration of cellular resistance to N-methyl-N′-nitro-N-nitrosoguanidine

Kanji Ishizaki, Tohru Tsujimura, Hideo Yawata, Chikau Fujio, Yusaku Nakabeppu, Mutsuo Sekiguchi, Mituo Ikenaga

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

We have constructed a plasmid on which the E. coli O6-methylguanine-DNA methyltransferase (MT) gene (ada gene) was linked with an SV40 promoter sequence and a poly(A) site. After transferring this plasmid into Mer- HeLa MR cells by DNA transfection, effective expression of E. coli MT was observed. Isolated stable transformant clones showed higher resistance to N-methyl-N′-nitro-N-nitrosoguanidine in colony formation and sister-chromatid exchange induction than HeLa MR cells.

Original languageEnglish
Pages (from-to)135-141
Number of pages7
JournalMutation Research DNA Repair Reports
Volume166
Issue number2
DOIs
Publication statusPublished - Sep 1986

All Science Journal Classification (ASJC) codes

  • Medicine(all)

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