Transfer of the E. coli O6-methyltransferase gene into repair-deficient human cells and restoration of cellular resistance to N-methyl-N′-nitro-N-nitrosoguanidine

Kanji Ishizaki, Tohru Tsujimura, Hideo Yawata, Chikau Fujio, Yusaku Nakabeppu, Mutsuo Sekiguchi, Mituo Ikenaga

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

We have constructed a plasmid on which the E. coli O6-methylguanine-DNA methyltransferase (MT) gene (ada gene) was linked with an SV40 promoter sequence and a poly(A) site. After transferring this plasmid into Mer- HeLa MR cells by DNA transfection, effective expression of E. coli MT was observed. Isolated stable transformant clones showed higher resistance to N-methyl-N′-nitro-N-nitrosoguanidine in colony formation and sister-chromatid exchange induction than HeLa MR cells.

Original languageEnglish
Pages (from-to)135-141
Number of pages7
JournalMutation Research DNA Repair Reports
Volume166
Issue number2
DOIs
Publication statusPublished - Sep 1986

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Methylnitronitrosoguanidine
Methyltransferases
HeLa Cells
Plasmids
Escherichia coli
Sister Chromatid Exchange
Poly A
Genes
Transfection
Clone Cells
DNA
E coli Ada protein

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Transfer of the E. coli O6-methyltransferase gene into repair-deficient human cells and restoration of cellular resistance to N-methyl-N′-nitro-N-nitrosoguanidine. / Ishizaki, Kanji; Tsujimura, Tohru; Yawata, Hideo; Fujio, Chikau; Nakabeppu, Yusaku; Sekiguchi, Mutsuo; Ikenaga, Mituo.

In: Mutation Research DNA Repair Reports, Vol. 166, No. 2, 09.1986, p. 135-141.

Research output: Contribution to journalArticle

Ishizaki, Kanji ; Tsujimura, Tohru ; Yawata, Hideo ; Fujio, Chikau ; Nakabeppu, Yusaku ; Sekiguchi, Mutsuo ; Ikenaga, Mituo. / Transfer of the E. coli O6-methyltransferase gene into repair-deficient human cells and restoration of cellular resistance to N-methyl-N′-nitro-N-nitrosoguanidine. In: Mutation Research DNA Repair Reports. 1986 ; Vol. 166, No. 2. pp. 135-141.
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