Transfer of the E. coli O6-methyltransferase gene into repair-deficient human cells and restoration of cellular resistance to N-methyl-N′-nitro-N-nitrosoguanidine

Kanji Ishizaki; Tohru Tsujimura; Hideo Yawata; Chikau Fujio; Yusaku Nakabeppu; Mutsuo Sekiguchi; Mituo Ikenaga

doi:10.1016/0167-8817(86)90011-8

Transfer of the E. coli O⁶-methyltransferase gene into repair-deficient human cells and restoration of cellular resistance to N-methyl-N′-nitro-N-nitrosoguanidine

Kanji Ishizaki, Tohru Tsujimura, Hideo Yawata, Chikau Fujio, Yusaku Nakabeppu, Mutsuo Sekiguchi, Mituo Ikenaga

Research output: Contribution to journal › Article › peer-review

52 Citations (Scopus)

Abstract

We have constructed a plasmid on which the E. coli O⁶-methylguanine-DNA methyltransferase (MT) gene (ada gene) was linked with an SV40 promoter sequence and a poly(A) site. After transferring this plasmid into Mer^- HeLa MR cells by DNA transfection, effective expression of E. coli MT was observed. Isolated stable transformant clones showed higher resistance to N-methyl-N′-nitro-N-nitrosoguanidine in colony formation and sister-chromatid exchange induction than HeLa MR cells.

Original language	English
Pages (from-to)	135-141
Number of pages	7
Journal	Mutation Research DNA Repair Reports
Volume	166
Issue number	2
DOIs	https://doi.org/10.1016/0167-8817(86)90011-8
Publication status	Published - Sept 1986
Externally published	Yes

All Science Journal Classification (ASJC) codes

Medicine(all)

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10.1016/0167-8817(86)90011-8

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Transfer of the E. coli O⁶-methyltransferase gene into repair-deficient human cells and restoration of cellular resistance to N-methyl-N′-nitro-N-nitrosoguanidine. / Ishizaki, Kanji; Tsujimura, Tohru; Yawata, Hideo et al.
In: Mutation Research DNA Repair Reports, Vol. 166, No. 2, 09.1986, p. 135-141.

Research output: Contribution to journal › Article › peer-review

Ishizaki, K, Tsujimura, T, Yawata, H, Fujio, C, Nakabeppu, Y, Sekiguchi, M & Ikenaga, M 1986, 'Transfer of the E. coli O⁶-methyltransferase gene into repair-deficient human cells and restoration of cellular resistance to N-methyl-N′-nitro-N-nitrosoguanidine', Mutation Research DNA Repair Reports, vol. 166, no. 2, pp. 135-141. https://doi.org/10.1016/0167-8817(86)90011-8

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title = "Transfer of the E. coli O6-methyltransferase gene into repair-deficient human cells and restoration of cellular resistance to N-methyl-N′-nitro-N-nitrosoguanidine",

abstract = "We have constructed a plasmid on which the E. coli O6-methylguanine-DNA methyltransferase (MT) gene (ada gene) was linked with an SV40 promoter sequence and a poly(A) site. After transferring this plasmid into Mer- HeLa MR cells by DNA transfection, effective expression of E. coli MT was observed. Isolated stable transformant clones showed higher resistance to N-methyl-N′-nitro-N-nitrosoguanidine in colony formation and sister-chromatid exchange induction than HeLa MR cells.",

author = "Kanji Ishizaki and Tohru Tsujimura and Hideo Yawata and Chikau Fujio and Yusaku Nakabeppu and Mutsuo Sekiguchi and Mituo Ikenaga",

note = "Funding Information: This work was supported by Grants for Cancer Research from the Ministry of Education, Science and Culture, Japan. One of the authors (Y.N.) was a Visiting Research Fellow in Cancer Research at the Radiation Biology Center, Kyoto University, supported by the Japanese Society for the Promotion of Science.",

year = "1986",

month = sep,

doi = "10.1016/0167-8817(86)90011-8",

language = "English",

volume = "166",

pages = "135--141",

journal = "Mutation Research DNA Repair Reports",

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T1 - Transfer of the E. coli O6-methyltransferase gene into repair-deficient human cells and restoration of cellular resistance to N-methyl-N′-nitro-N-nitrosoguanidine

AU - Ishizaki, Kanji

AU - Tsujimura, Tohru

AU - Yawata, Hideo

AU - Fujio, Chikau

AU - Nakabeppu, Yusaku

AU - Sekiguchi, Mutsuo

AU - Ikenaga, Mituo

N1 - Funding Information: This work was supported by Grants for Cancer Research from the Ministry of Education, Science and Culture, Japan. One of the authors (Y.N.) was a Visiting Research Fellow in Cancer Research at the Radiation Biology Center, Kyoto University, supported by the Japanese Society for the Promotion of Science.

PY - 1986/9

Y1 - 1986/9

N2 - We have constructed a plasmid on which the E. coli O6-methylguanine-DNA methyltransferase (MT) gene (ada gene) was linked with an SV40 promoter sequence and a poly(A) site. After transferring this plasmid into Mer- HeLa MR cells by DNA transfection, effective expression of E. coli MT was observed. Isolated stable transformant clones showed higher resistance to N-methyl-N′-nitro-N-nitrosoguanidine in colony formation and sister-chromatid exchange induction than HeLa MR cells.

AB - We have constructed a plasmid on which the E. coli O6-methylguanine-DNA methyltransferase (MT) gene (ada gene) was linked with an SV40 promoter sequence and a poly(A) site. After transferring this plasmid into Mer- HeLa MR cells by DNA transfection, effective expression of E. coli MT was observed. Isolated stable transformant clones showed higher resistance to N-methyl-N′-nitro-N-nitrosoguanidine in colony formation and sister-chromatid exchange induction than HeLa MR cells.

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Transfer of the E. coli O⁶-methyltransferase gene into repair-deficient human cells and restoration of cellular resistance to N-methyl-N′-nitro-N-nitrosoguanidine

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