TY - JOUR
T1 - Transformation of
T2 - Pueraria candollei var. mirifica phytoestrogens using immobilized and free β-glucosidase, a technique for enhancing estrogenic activity
AU - Makkliang, Fonthip
AU - Juengsanguanpornsuk, Wipawee
AU - Phaisan, Suppalak
AU - Sakdamas, Attapon
AU - Putalun, Waraporn
AU - Sakamoto, Seiichi
AU - Yusakul, Gorawit
N1 - Funding Information:
This work was financially supported by the Agricultural Research Development Agency (Public Organization) (grant number: CRP6305030030). The authors would also like to thank the Center for Scientific and Technological Equipment of Walailak University for providing all facilities and equipment. Partially, this research was financially supported by the new strategic research project (P2P), Walailak University, Thailand.
Funding Information:
This work was nancially supported by the Agricultural Research Development Agency (Public Organization) (grant number: CRP6305030030). The authors would also like to thank the Center for Scientic and Technological Equipment of Walailak University for providing all facilities and equipment. Partially, this research was nancially supported by the new strategic research project (P2P), Walailak University, Thailand.
Publisher Copyright:
© The Royal Society of Chemistry.
PY - 2021/9/23
Y1 - 2021/9/23
N2 - Pueraria candollei var. mirifica (PM) has a significant beneficial effect on postmenopausal symptoms associated with estrogen deficiency. However, the estrogenic activity and intestinal absorption of isoflavonoid glycosides derived from PM, such as daidzin and genistin, are significantly lower than those of their aglycones. To enhance the estrogenic activity of the PM extract, we developed β-glucosidase and its immobilized form to increase the PM aglycone content (daidzein and genistein). The enzyme immobilization was done by alginate beads, and the resulting β-glucosidase alginate beads have a diameter of about 0.20 cm. Response surface methodology (RSM) was used to optimize certain parameters, such as the pH, temperature, and ethanol concentration. The optimal conditions of β-glucosidase for daidzein and genistein production were pH of 4.8-4.9, a temperature in the range 46.3-49.1 °C, and ethanol concentration of 10.0-11.0%. The ANOVA results indicated that the design experiment involving free and immobilized β-glucosidase was the best fit by quadratic models, which had adjusted R2 values between 0.8625 and 0.9318. Immobilized β-glucosidase can be reused up to nine times and maintained efficacy of greater than 90%. Treatment of the PM extract with β-glucosidase increased the estrogenic activity of the PM extract by 8.71- to 23.2-fold compared to that of the untreated extract. Thus, β-glucosidase has a high potential for enhancing the estrogenic activity of PM constituents, and it can be applied on an industrial scale to increase the utility of these natural products. This journal is
AB - Pueraria candollei var. mirifica (PM) has a significant beneficial effect on postmenopausal symptoms associated with estrogen deficiency. However, the estrogenic activity and intestinal absorption of isoflavonoid glycosides derived from PM, such as daidzin and genistin, are significantly lower than those of their aglycones. To enhance the estrogenic activity of the PM extract, we developed β-glucosidase and its immobilized form to increase the PM aglycone content (daidzein and genistein). The enzyme immobilization was done by alginate beads, and the resulting β-glucosidase alginate beads have a diameter of about 0.20 cm. Response surface methodology (RSM) was used to optimize certain parameters, such as the pH, temperature, and ethanol concentration. The optimal conditions of β-glucosidase for daidzein and genistein production were pH of 4.8-4.9, a temperature in the range 46.3-49.1 °C, and ethanol concentration of 10.0-11.0%. The ANOVA results indicated that the design experiment involving free and immobilized β-glucosidase was the best fit by quadratic models, which had adjusted R2 values between 0.8625 and 0.9318. Immobilized β-glucosidase can be reused up to nine times and maintained efficacy of greater than 90%. Treatment of the PM extract with β-glucosidase increased the estrogenic activity of the PM extract by 8.71- to 23.2-fold compared to that of the untreated extract. Thus, β-glucosidase has a high potential for enhancing the estrogenic activity of PM constituents, and it can be applied on an industrial scale to increase the utility of these natural products. This journal is
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U2 - 10.1039/d1ra05109a
DO - 10.1039/d1ra05109a
M3 - Article
AN - SCOPUS:85120000962
VL - 11
SP - 32067
EP - 32076
JO - RSC Advances
JF - RSC Advances
SN - 2046-2069
IS - 51
ER -