Transformation of silage-making Lactobacillus strains by electroporation with plasmid vectors

Guiwen Fan, Eiji Takahashi, Katsumi Doi, Shorin Matsuo, Osamu Tanaka, Sadahiro Ohmomo, Seiya Ogata

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Several newly isolated strains of Lactobacillus for silage fermentation were transformed with plasmid vectors, pGK12 and pSA3, by electroporation. Transformation efficiency depended on the electric field strength, time-constant, polyethyleneglycol (PEG) molecular weight in electroporation buffer and the incubation temperature after exposition of pulses. Transformation of L. pentosus NGRI0225 resulted in the maximum transformation efficiency of 5.7 × 105 transformants perμg DNA of pGK12 with an electric field strength of 7.0kV/cm, time-constant of 6.2 ms and 30% (W/V) of PEG 1000. Plasmid DNAs isolated from all transformants did not show any detectable rearrangements or deletions under these conditions.

Original languageEnglish
Pages (from-to)217-225
Number of pages9
JournalJournal of the Faculty of Agriculture, Kyushu University
Volume43
Issue number1-2
Publication statusPublished - Nov 1998

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science

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