Several newly isolated strains of Lactobacillus for silage fermentation were transformed with plasmid vectors, pGK12 and pSA3, by electroporation. Transformation efficiency depended on the electric field strength, time-constant, polyethyleneglycol (PEG) molecular weight in electroporation buffer and the incubation temperature after exposition of pulses. Transformation of L. pentosus NGRI0225 resulted in the maximum transformation efficiency of 5.7 × 105 transformants perμg DNA of pGK12 with an electric field strength of 7.0kV/cm, time-constant of 6.2 ms and 30% (W/V) of PEG 1000. Plasmid DNAs isolated from all transformants did not show any detectable rearrangements or deletions under these conditions.
|Number of pages||9|
|Journal||Journal of the Faculty of Agriculture, Kyushu University|
|Publication status||Published - Nov 1998|
All Science Journal Classification (ASJC) codes
- Agronomy and Crop Science