Transformation of silage-making Lactobacillus strains by electroporation with plasmid vectors

Guiwen Fan, Eiji Takahashi, Katsumi Doi, Shorin Matsuo, Osamu Tanaka, Sadahiro Ohmomo, Seiya Ogata

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


Several newly isolated strains of Lactobacillus for silage fermentation were transformed with plasmid vectors, pGK12 and pSA3, by electroporation. Transformation efficiency depended on the electric field strength, time-constant, polyethyleneglycol (PEG) molecular weight in electroporation buffer and the incubation temperature after exposition of pulses. Transformation of L. pentosus NGRI0225 resulted in the maximum transformation efficiency of 5.7 × 105 transformants perμg DNA of pGK12 with an electric field strength of 7.0kV/cm, time-constant of 6.2 ms and 30% (W/V) of PEG 1000. Plasmid DNAs isolated from all transformants did not show any detectable rearrangements or deletions under these conditions.

Original languageEnglish
Pages (from-to)217-225
Number of pages9
JournalJournal of the Faculty of Agriculture, Kyushu University
Issue number1-2
Publication statusPublished - Nov 1998

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science


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