TY - JOUR
T1 - Transformation of temperature-sensitive growth mutant of BHK21 cell line to wild-type phenotype with hamster and mouse DNA
AU - Kai, Ryosuke
AU - Sekiguchi, Takeshi
AU - Yamashita, Katsumi
AU - Sekiguchi, Mutsuo
AU - Nishimoto, Takeharu
PY - 1983/11/1
Y1 - 1983/11/1
N2 - A temperature-sensitive (ts) mutant, tsBN2, which was derived from BHK21 and is defective in the regulatory mechanism for chromosome condensation, was transformed to the temperature-resistant (ts+) phenotype by means of DNA-mediated gene transfer with hamster and mouse DNA. Treatment of mouse DNA with the restriction enzymes EcoRI, HindIII, PstI and Sall, but not with XhoI, almost completely abolished the transforming activity. A fluctuation test, originally devised by Luria and Delbrück, was used to estimate the reversion and transformation frequencies of tsBN2 cultures.
AB - A temperature-sensitive (ts) mutant, tsBN2, which was derived from BHK21 and is defective in the regulatory mechanism for chromosome condensation, was transformed to the temperature-resistant (ts+) phenotype by means of DNA-mediated gene transfer with hamster and mouse DNA. Treatment of mouse DNA with the restriction enzymes EcoRI, HindIII, PstI and Sall, but not with XhoI, almost completely abolished the transforming activity. A fluctuation test, originally devised by Luria and Delbrück, was used to estimate the reversion and transformation frequencies of tsBN2 cultures.
UR - http://www.scopus.com/inward/record.url?scp=0021047749&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0021047749&partnerID=8YFLogxK
U2 - 10.1007/BF01539472
DO - 10.1007/BF01539472
M3 - Article
C2 - 6318373
AN - SCOPUS:0021047749
SN - 0098-0366
VL - 9
SP - 673
EP - 680
JO - Somatic Cell Genetics
JF - Somatic Cell Genetics
IS - 6
ER -