Transformation of temperature-sensitive growth mutant of BHK21 cell line to wild-type phenotype with hamster and mouse DNA

Ryosuke Kai, Takeshi Sekiguchi, Katsumi Yamashita, Mutsuo Sekiguchi, Takeharu Nishimoto

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

A temperature-sensitive (ts) mutant, tsBN2, which was derived from BHK21 and is defective in the regulatory mechanism for chromosome condensation, was transformed to the temperature-resistant (ts+) phenotype by means of DNA-mediated gene transfer with hamster and mouse DNA. Treatment of mouse DNA with the restriction enzymes EcoRI, HindIII, PstI and Sall, but not with XhoI, almost completely abolished the transforming activity. A fluctuation test, originally devised by Luria and Delbrück, was used to estimate the reversion and transformation frequencies of tsBN2 cultures.

Original languageEnglish
Pages (from-to)673-680
Number of pages8
JournalSomatic Cell Genetics
Volume9
Issue number6
DOIs
Publication statusPublished - Nov 1 1983

All Science Journal Classification (ASJC) codes

  • Genetics

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