Transforming growth factor-β in the brain regulates fat metabolism during endurance exercise

Toma Ishikawa, Wataru Mizunoya, Tetsuro Shibakusa, Kazuo Inoue, Tohru Fushiki

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

We have previously reported that the concentration of transforming growth factor-β (TGF-β) increases in the cerebrospinal fluid of rats during exercise and that there is an increase in whole body fat oxidation following the intracisternal administration of TGF-β. These results led us to postulate that TGF-β in the brain regulates the enhancement of fatty acid oxidation during exercise. To test this hypothesis, we carried out respiratory gas analysis during treadmill running following the inhibition of TGF-β activity in rat brain by intracisternal administration of anti-TGF-β antibody or SB-431542, an inhibitor of the type 1 TGF-β receptor. We found that each reagent partially blocked the increase in the fatty acid oxidation. We also compared the plasma concentrations of energy substrates in the group administered anti-TGF-β antibody and the control group during running. We found that the plasma concentrations of nonesterified fatty acids and ketone bodies in the group administered anti-TGF-β antibody were lower than in the control group at the end of running. In the same way, we carried out respiratory gas analysis during treadmill running after depressing corticotropin-releasing factor activity in the brain using intracisternal administration of astressin, an inhibitor of the corticotropin-releasing factor receptor. However, there were no significant differences in respiratory exchange ratio or oxygen consumption in moderate running (60% maximum oxygen consumption). These results suggest that brain TGF-β has a role in enhancing fatty acid oxidation during endurance exercise and that this regulation is executed at least partly via the type 1 TGF-β receptor signal transduction system.

Original languageEnglish
Pages (from-to)E1151-E1159
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume291
Issue number6
DOIs
Publication statusPublished - 2006

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Physiology (medical)

Fingerprint Dive into the research topics of 'Transforming growth factor-β in the brain regulates fat metabolism during endurance exercise'. Together they form a unique fingerprint.

Cite this