Transforming growth factor β1 promotes migration of human periodontal ligament cells through heat shock protein 27 phosphorylation

Seong Min Kwon, Soo A. Kim, Shinsuke Fujii, Hidefumi Maeda, Sang Gun Ahn, Jung Hoon Yoon

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Transforming growth factor β1 (TGF- β1) regulates cellular functions including cell growth, differentiation, and migration. However, signal transduction pathways of TGF- β1 are mostly undefined in human periodontal ligament (hPDL) cells. In this study, we investigated TGF β1-induced migration focusing on heat shock protein 27 (Hsp27) activation. The cellular response to TGF- β1 by hPDL cells was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and Western blot analysis. Cell migration was determined by counting migrated cells using a Chemotaxis Cell Migration Assay kit. TGF- β1 induced cell migration and increased the phosphorylation of Hsp27 and p38 mitogen-activated protein kinase (MAPK) in hPDL cells. Inhibiting p38 MAPK with SB203580 blocked TGF- β1-induced Hsp27 activation and cell migration. These findings suggest that TGF- β1-induced Hsp27 may promote the migration of hPDL cells via p38 MAPK mediated signaling. Hsp27 may be a potential target for the periodontal regeneration process related to cell migration.

Original languageEnglish
Pages (from-to)486-489
Number of pages4
JournalBiological and Pharmaceutical Bulletin
Volume34
Issue number4
DOIs
Publication statusPublished - Apr 2011

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science

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