Transgene overexpression with cognate small interfering RNA in tobacco

Rie Tomita, Tatsurou Hamada, Gorou Horiguchi, Koh Iba, Hiroaki Kodama

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Small interfering RNAs (siRNAs) are a key component of RNA silencing, including cosuppression. Here, we show an example in which siRNA does not serve in the downregulation of target genes. A tobacco endoplasmic reticulum ω-3 fatty acid desaturase (NtFAD3) catalyzes the formation of α-linolenate (18:3). Introduction of the NtFAD3 gene into tobacco plants caused strong reduction of 18:3 content in leaf tissues, which is associated with the production of the NtFAD3 siRNAs. However, this silencing effect was lacking in the root tissues. Both the introduced NtFAD3 and endogenous NtFAD3 genes were expressed successfully, and the roots showed increased 18:3 phenotype. Surprisingly, the NtFAD3 siRNAs were produced even in the root tissues. Expression of a hairpin double-stranded RNA against the NtFAD3 gene caused efficient reduction of 18:3 content in root tissues. Therefore, cosuppression of the NtFAD3 gene in tobacco appears to include an as yet unidentified developmental stage and tissue-specific mechanism of regulation of siRNA function.

Original languageEnglish
Pages (from-to)117-120
Number of pages4
JournalFEBS Letters
Volume573
Issue number1-3
DOIs
Publication statusPublished - Aug 27 2004

Fingerprint

Tobacco
Transgenes
Small Interfering RNA
Genes
Tissue
RNA Interference
Fatty Acid Desaturases
alpha-Linolenic Acid
Double-Stranded RNA
Endoplasmic Reticulum
Down-Regulation
RNA
Phenotype

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Transgene overexpression with cognate small interfering RNA in tobacco. / Tomita, Rie; Hamada, Tatsurou; Horiguchi, Gorou; Iba, Koh; Kodama, Hiroaki.

In: FEBS Letters, Vol. 573, No. 1-3, 27.08.2004, p. 117-120.

Research output: Contribution to journalArticle

Tomita, R, Hamada, T, Horiguchi, G, Iba, K & Kodama, H 2004, 'Transgene overexpression with cognate small interfering RNA in tobacco', FEBS Letters, vol. 573, no. 1-3, pp. 117-120. https://doi.org/10.1016/j.febslet.2004.07.063
Tomita, Rie ; Hamada, Tatsurou ; Horiguchi, Gorou ; Iba, Koh ; Kodama, Hiroaki. / Transgene overexpression with cognate small interfering RNA in tobacco. In: FEBS Letters. 2004 ; Vol. 573, No. 1-3. pp. 117-120.
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