Transgenic analysis of the BmBLOS2 gene that governs the translucency of the larval integument of the silkworm, Bombyx mori

T. Fujii, T. Daimon, K. Uchino, Y. Banno, S. Katsuma, H. Sezutsu, T. Tamura, T. Shimada

Research output: Contribution to journalArticle

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Abstract

The larval integument of the silkworm, Bombyx mori, is opaque because urate granules accumulate in the epidermis. Although the biosynthetic pathway of uric acid is well studied, little is known about how uric acid accumulates as urate granules in epidermal cells. In the distinct oily (od) mutant silkworm, the larval integument is translucent because of the inability to construct urate granules. Recently, we have found that the od mutant has a genomic deletion in the B. mori homologue of the human biogenesis of lysosome-related organelles complex1, subunit 2 (BLOS2) gene (BmBLOS2). Here, we performed a molecular and functional characterization of BmBLOS2. Northern blot analysis showed that BmBLOS2 was ubiquitously expressed in various tissues. We analysed the structure of a newly isolated mutant (odB) allelic to od and found a premature stop codon in the coding sequence of BmBLOS2 in this new mutation. Moreover, the translucent phenotype was rescued by the germ-line transformation of the wild-type BmBLOS2 allele into the od mutant. Our results suggest that BmBLOS2 is responsible for the od mutant phenotype and plays a crucial role in biogenesis of urate granules in the larval epidermis of the silkworm. The relationships amongst Hermansky-Pudlak syndrome (HPS) genes in mammals, granule group genes in Drosophila and translucent mutant genes in B. mori are discussed.

Original languageEnglish
Pages (from-to)659-667
Number of pages9
JournalInsect Molecular Biology
Volume19
Issue number5
DOIs
Publication statusPublished - Oct 1 2010

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Bombyx
Bombyx mori
silkworms
Uric Acid
integument
genetically modified organisms
granules
mutants
Genes
genes
epidermis (animal)
uric acid
Epidermis
Hermanski-Pudlak Syndrome
Phenotype
phenotype
stop codon
Nonsense Codon
Biosynthetic Pathways
lysosomes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Insect Science

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Transgenic analysis of the BmBLOS2 gene that governs the translucency of the larval integument of the silkworm, Bombyx mori. / Fujii, T.; Daimon, T.; Uchino, K.; Banno, Y.; Katsuma, S.; Sezutsu, H.; Tamura, T.; Shimada, T.

In: Insect Molecular Biology, Vol. 19, No. 5, 01.10.2010, p. 659-667.

Research output: Contribution to journalArticle

Fujii, T. ; Daimon, T. ; Uchino, K. ; Banno, Y. ; Katsuma, S. ; Sezutsu, H. ; Tamura, T. ; Shimada, T. / Transgenic analysis of the BmBLOS2 gene that governs the translucency of the larval integument of the silkworm, Bombyx mori. In: Insect Molecular Biology. 2010 ; Vol. 19, No. 5. pp. 659-667.
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