TY - JOUR
T1 - Transgenic mice expressing a soluble form of porcine nectin-1/herpesvirus entry mediator C as a model for pseudorabies-resistant livestock
AU - Ono, Etsuro
AU - Amagai, Keiko
AU - Taharaguchi, Satoshi
AU - Tomioka, Yukiko
AU - Yoshino, Saori
AU - Watanabe, Yuki
AU - Cherel, Pierre
AU - Houdebine, Louis Marie
AU - Adam, Micheline
AU - Eloit, Marc
AU - Inobe, Manabu
AU - Uede, Toshimitsu
PY - 2004/11/16
Y1 - 2004/11/16
N2 - An approach to genetically engineered resistance to pseudorabies virus (PRV) infection was examined by using a transgene encoding a soluble form of nectin-1, also known as herpesvirus entry mediator C. Nectin-1 is an α-herpesvirus receptor that binds to virion glycoprotein D. Nectin-1 mediates entry of PRV, herpes simplex virus types 1 and 2, and bovine herpesvirus type 1. To assess the antiviral potential of an ectopic expression of the nectin-1 ectodomain in vivo, six transgenic mouse lines expressing a soluble form of nectin-1, consisting of an extracellular domain of porcine nectin-1 and the Fc portion of human IgGI, were generated. All of the transgenic mouse lines showed nearly complete resistance to PRV infection by means of both i.p. and intranasal routes. These results suggest that the introduction into farm animals of a transgene encoding a soluble form of nectin-1 would offer a potent biological approach to generating α-herpesvirus-resistant livestock.
AB - An approach to genetically engineered resistance to pseudorabies virus (PRV) infection was examined by using a transgene encoding a soluble form of nectin-1, also known as herpesvirus entry mediator C. Nectin-1 is an α-herpesvirus receptor that binds to virion glycoprotein D. Nectin-1 mediates entry of PRV, herpes simplex virus types 1 and 2, and bovine herpesvirus type 1. To assess the antiviral potential of an ectopic expression of the nectin-1 ectodomain in vivo, six transgenic mouse lines expressing a soluble form of nectin-1, consisting of an extracellular domain of porcine nectin-1 and the Fc portion of human IgGI, were generated. All of the transgenic mouse lines showed nearly complete resistance to PRV infection by means of both i.p. and intranasal routes. These results suggest that the introduction into farm animals of a transgene encoding a soluble form of nectin-1 would offer a potent biological approach to generating α-herpesvirus-resistant livestock.
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U2 - 10.1073/pnas.0405816101
DO - 10.1073/pnas.0405816101
M3 - Article
C2 - 15534229
AN - SCOPUS:9244233815
SN - 0027-8424
VL - 101
SP - 16150
EP - 16155
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 46
ER -