Transgenic mice expressing a soluble form of porcine nectin-1/herpesvirus entry mediator C as a model for pseudorabies-resistant livestock

Etsuro Ono, Keiko Amagai, Satoshi Taharaguchi, Yukiko Tomioka, Saori Yoshino, Yuki Watanabe, Pierre Cherel, Louis Marie Houdebine, Micheline Adam, Marc Eloit, Manabu Inobe, Toshimitsu Uede

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30 Citations (Scopus)

Abstract

An approach to genetically engineered resistance to pseudorabies virus (PRV) infection was examined by using a transgene encoding a soluble form of nectin-1, also known as herpesvirus entry mediator C. Nectin-1 is an α-herpesvirus receptor that binds to virion glycoprotein D. Nectin-1 mediates entry of PRV, herpes simplex virus types 1 and 2, and bovine herpesvirus type 1. To assess the antiviral potential of an ectopic expression of the nectin-1 ectodomain in vivo, six transgenic mouse lines expressing a soluble form of nectin-1, consisting of an extracellular domain of porcine nectin-1 and the Fc portion of human IgGI, were generated. All of the transgenic mouse lines showed nearly complete resistance to PRV infection by means of both i.p. and intranasal routes. These results suggest that the introduction into farm animals of a transgene encoding a soluble form of nectin-1 would offer a potent biological approach to generating α-herpesvirus-resistant livestock.

Original languageEnglish
Pages (from-to)16150-16155
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume101
Issue number46
DOIs
Publication statusPublished - Nov 16 2004
Externally publishedYes

All Science Journal Classification (ASJC) codes

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