Translation initiation: A regulatory role for poly(A) tracts in front of the AUG codon in saccharomyces cerevisiae

Xuhua Xia, Vivian Mackay, Xiaoquan Yao, Jianhua Wu, Fumihito Miura, Takashi Ito, David R. Morris

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The 59-UTR serves as the loading dock for ribosomes during translation initiation and is the key site for translation regulation. Many genes in the yeast Saccharomyces cerevisiae contain poly(A) tracts in their 59-UTRs. We studied these pre-AUG poly(A) tracts in a set of 3274 recently identified 59-UTRs in the yeast to characterize their effect on in vivo protein abundance, ribosomal density, and protein synthesis rate in the yeast. The protein abundance and the protein synthesis rate increase with the length of the poly(A), but exhibit a dramatic decrease when the poly(A) length is ≥12. The ribosomal density also reaches the lowest level when the poly(A) length is ≥12. This supports the hypothesis that a pre-AUG poly(A) tract can bind to translation initiation factors to enhance translation initiation, but a long (≥12) pre-AUG poly(A) tract will bind to Pab1p, whose binding size is 12 consecutive A residues in yeast, resulting in repression of translation. The hypothesis explains why a long pre-AUG poly(A) leads to more efficient translation initiation than a short one when PABP is absent, and why pre-AUG poly(A) is short in the early genes but long in the late genes of vaccinia virus.

Original languageEnglish
Pages (from-to)469-478
Number of pages10
JournalGenetics
Volume189
Issue number2
DOIs
Publication statusPublished - Oct 1 2011
Externally publishedYes

Fingerprint

Poly A
Codon
Saccharomyces cerevisiae
Untranslated Regions
Yeasts
Genes
Peptide Initiation Factors
Proteins
Vaccinia virus
Protein Biosynthesis
Ribosomes

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Translation initiation : A regulatory role for poly(A) tracts in front of the AUG codon in saccharomyces cerevisiae. / Xia, Xuhua; Mackay, Vivian; Yao, Xiaoquan; Wu, Jianhua; Miura, Fumihito; Ito, Takashi; Morris, David R.

In: Genetics, Vol. 189, No. 2, 01.10.2011, p. 469-478.

Research output: Contribution to journalArticle

Xia, Xuhua ; Mackay, Vivian ; Yao, Xiaoquan ; Wu, Jianhua ; Miura, Fumihito ; Ito, Takashi ; Morris, David R. / Translation initiation : A regulatory role for poly(A) tracts in front of the AUG codon in saccharomyces cerevisiae. In: Genetics. 2011 ; Vol. 189, No. 2. pp. 469-478.
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