Translation initiation from non-AUG codons in cos1 cells is mRNA species dependent

Kailash C. Gupta, Etsuro Ono, Edgardo V. Ariztia, Mutsumi Inaba

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Sendai virus P/C mRNA, human erythrocyte membrane protein 4.1 mRNA and PDGF-B chain mRNA were used to test whether translation initiation from non-AUG codons in COS1 cells was mRNA species dependent. Site-directed mutants of the authentic translation start sites of these mRNAs to alternate start codons showed that while P/C mRNA is capable of initiating translation from non-AUG start sites the other two mRNAs are not. Our study shows that translation initiation from non-AUG codons is mRNA species dependent and suggests that higher order structure of an mRNA determines the non-AUG translation start site.

Original languageEnglish
Pages (from-to)567-573
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume201
Issue number2
DOIs
Publication statusPublished - Jun 15 1994

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Codon
Messenger RNA
Sendai virus
Initiator Codon
Erythrocyte Membrane
Viruses
Membrane Proteins

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Translation initiation from non-AUG codons in cos1 cells is mRNA species dependent. / Gupta, Kailash C.; Ono, Etsuro; Ariztia, Edgardo V.; Inaba, Mutsumi.

In: Biochemical and Biophysical Research Communications, Vol. 201, No. 2, 15.06.1994, p. 567-573.

Research output: Contribution to journalArticle

Gupta, Kailash C. ; Ono, Etsuro ; Ariztia, Edgardo V. ; Inaba, Mutsumi. / Translation initiation from non-AUG codons in cos1 cells is mRNA species dependent. In: Biochemical and Biophysical Research Communications. 1994 ; Vol. 201, No. 2. pp. 567-573.
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