Translation initiation from non-AUG codons in cos1 cells is mRNA species dependent

Kailash C. Gupta, Etsuro Ono, Edgardo V. Ariztia, Mutsumi Inaba

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Sendai virus P/C mRNA, human erythrocyte membrane protein 4.1 mRNA and PDGF-B chain mRNA were used to test whether translation initiation from non-AUG codons in COS1 cells was mRNA species dependent. Site-directed mutants of the authentic translation start sites of these mRNAs to alternate start codons showed that while P/C mRNA is capable of initiating translation from non-AUG start sites the other two mRNAs are not. Our study shows that translation initiation from non-AUG codons is mRNA species dependent and suggests that higher order structure of an mRNA determines the non-AUG translation start site.

Original languageEnglish
Pages (from-to)567-573
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume201
Issue number2
DOIs
Publication statusPublished - Jun 15 1994
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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