Translational control by secondary-structure formation in mRNA in a eukaryotic system

Daisuke Kawaguchi, Saaya Shimizu, Naoko Abe, Fumitaka Hashiya, Fumiaki Tomoike, Yasuaki Kimura, Hiroshi Abe

Research output: Contribution to journalArticle

Abstract

Eukaryotic mRNA has a cap structure at the 5′ end and a poly(A) tail at the 3′ end. The cap and poly(A) tail form a complex with multiple translation factors, and mRNA forms a circularized structure called a closed-loop model. This circularized structure reportedly not only stabilizes mRNA but also promotes ribosome recycling during translation, which improves translation efficiency. We designed an artificial mRNA that forms a circularized structure without a cap structure and poly(A) tail and found that its translational efficiency was improved compared with that of a sequence without the circularized structure in a eukaryotic translation system.

Original languageEnglish
JournalNucleosides, Nucleotides and Nucleic Acids
DOIs
Publication statusAccepted/In press - Jan 1 2019

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Messenger RNA
Protein Biosynthesis
Ribosomes
Recycling

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Genetics

Cite this

Translational control by secondary-structure formation in mRNA in a eukaryotic system. / Kawaguchi, Daisuke; Shimizu, Saaya; Abe, Naoko; Hashiya, Fumitaka; Tomoike, Fumiaki; Kimura, Yasuaki; Abe, Hiroshi.

In: Nucleosides, Nucleotides and Nucleic Acids, 01.01.2019.

Research output: Contribution to journalArticle

Kawaguchi, Daisuke ; Shimizu, Saaya ; Abe, Naoko ; Hashiya, Fumitaka ; Tomoike, Fumiaki ; Kimura, Yasuaki ; Abe, Hiroshi. / Translational control by secondary-structure formation in mRNA in a eukaryotic system. In: Nucleosides, Nucleotides and Nucleic Acids. 2019.
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