TY - JOUR
T1 - Translational control by secondary-structure formation in mRNA in a eukaryotic system
AU - Kawaguchi, Daisuke
AU - Shimizu, Saaya
AU - Abe, Naoko
AU - Hashiya, Fumitaka
AU - Tomoike, Fumiaki
AU - Kimura, Yasuaki
AU - Abe, Hiroshi
N1 - Funding Information:
This work was supported by Japan Society for the Promotion of Science (JSPS) [Grant-in-Aid for Scientific Research (B), 16H04178 to H.A.; Grant-in-Aid for Early-Career Scientists, 18K14357 to N. A.] and CREST [JPMJCR18S1 to H.A.] from Japan Science and Technology Agency (JST). We thank Edanz (www.edanzediting.co.jp) for editing the English text of a draft of this manuscript.
Publisher Copyright:
© 2019, © 2019 Taylor & Francis Group, LLC.
PY - 2020/2/20
Y1 - 2020/2/20
N2 - Eukaryotic mRNA has a cap structure at the 5′ end and a poly(A) tail at the 3′ end. The cap and poly(A) tail form a complex with multiple translation factors, and mRNA forms a circularized structure called a closed-loop model. This circularized structure reportedly not only stabilizes mRNA but also promotes ribosome recycling during translation, which improves translation efficiency. We designed an artificial mRNA that forms a circularized structure without a cap structure and poly(A) tail and found that its translational efficiency was improved compared with that of a sequence without the circularized structure in a eukaryotic translation system.
AB - Eukaryotic mRNA has a cap structure at the 5′ end and a poly(A) tail at the 3′ end. The cap and poly(A) tail form a complex with multiple translation factors, and mRNA forms a circularized structure called a closed-loop model. This circularized structure reportedly not only stabilizes mRNA but also promotes ribosome recycling during translation, which improves translation efficiency. We designed an artificial mRNA that forms a circularized structure without a cap structure and poly(A) tail and found that its translational efficiency was improved compared with that of a sequence without the circularized structure in a eukaryotic translation system.
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U2 - 10.1080/15257770.2019.1671593
DO - 10.1080/15257770.2019.1671593
M3 - Article
C2 - 31566082
AN - SCOPUS:85073969147
SN - 1525-7770
VL - 39
SP - 195
EP - 203
JO - Nucleosides, Nucleotides and Nucleic Acids
JF - Nucleosides, Nucleotides and Nucleic Acids
IS - 1-3
ER -