Translational efficiency is up-regulated by alternative exon in murine IL-15 mRNA

Hitoshi Nishimura, Junji Washizu, Nobuhisa Nakamura, Atsushi Enomoto, Yasunobu Yoshikai

    Research output: Contribution to journalArticle

    66 Citations (Scopus)

    Abstract

    IL-15 promotes the growth of T cells and shares properties of IL-2. IL- 2 is produced exclusively by T cells, while IL-15 message is expressed by a variety of tissues. However, it has been difficult to demonstrate IL-15 in the supernatants of many cells that express message for this cytokine. This suggests that IL-15 production is regulated by post-transcriptional controls. In this study, we cloned three types of murine IL-15 cDNA isoforms generated by alternative splicing and compared the translational efficiency among these isoforms. The translational efficiency of isoforms with alternative exon 5 containing another 3' splice site was significantly higher than that of IL- 15 cDNA with originally described exon 5, which is generated by internal splicing of alternative exon 5. The translation product of the isoform containing alternative exon 5 has a shorter open reading frame due to stop codons in additional sequence, followed by a new AUG codon, and displays a shorter leader sequence. The shorter isoform of the IL-15 was detected in peritoneal macrophages stimulated with IFN-γ and LPS, which expressed an abundant level of alternative exon 5. These results suggest that normal IL- 15 production in stimulated macrophages is regulated by splicing of alternative exon 5.

    Original languageEnglish
    Pages (from-to)936-942
    Number of pages7
    JournalJournal of Immunology
    Volume160
    Issue number2
    Publication statusPublished - Jan 15 1998

    Fingerprint

    Interleukin-15
    Exons
    Messenger RNA
    Protein Isoforms
    Alternative Splicing
    Interleukin-2
    Complementary DNA
    T-Lymphocytes
    RNA Splice Sites
    Terminator Codon
    Peritoneal Macrophages
    Codon
    Open Reading Frames
    Macrophages
    Cytokines
    Growth

    All Science Journal Classification (ASJC) codes

    • Immunology and Allergy
    • Immunology

    Cite this

    Nishimura, H., Washizu, J., Nakamura, N., Enomoto, A., & Yoshikai, Y. (1998). Translational efficiency is up-regulated by alternative exon in murine IL-15 mRNA. Journal of Immunology, 160(2), 936-942.

    Translational efficiency is up-regulated by alternative exon in murine IL-15 mRNA. / Nishimura, Hitoshi; Washizu, Junji; Nakamura, Nobuhisa; Enomoto, Atsushi; Yoshikai, Yasunobu.

    In: Journal of Immunology, Vol. 160, No. 2, 15.01.1998, p. 936-942.

    Research output: Contribution to journalArticle

    Nishimura, H, Washizu, J, Nakamura, N, Enomoto, A & Yoshikai, Y 1998, 'Translational efficiency is up-regulated by alternative exon in murine IL-15 mRNA', Journal of Immunology, vol. 160, no. 2, pp. 936-942.
    Nishimura H, Washizu J, Nakamura N, Enomoto A, Yoshikai Y. Translational efficiency is up-regulated by alternative exon in murine IL-15 mRNA. Journal of Immunology. 1998 Jan 15;160(2):936-942.
    Nishimura, Hitoshi ; Washizu, Junji ; Nakamura, Nobuhisa ; Enomoto, Atsushi ; Yoshikai, Yasunobu. / Translational efficiency is up-regulated by alternative exon in murine IL-15 mRNA. In: Journal of Immunology. 1998 ; Vol. 160, No. 2. pp. 936-942.
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