Translational pausing ensures membrane targeting and cytoplasmic splicing of XBP1u mRNA

Kota Yanagitani, Yukio Kimata, Hiroshi Kadokura, Kenji Kohno

Research output: Contribution to journalArticle

104 Citations (Scopus)

Abstract

Upon endoplasmic reticulum (ER) stress, an endoribonuclease, inositol-requiring enzyme-1α, splices the precursor unspliced form of X-box-binding protein 1 messenger RNA (XBP1u mRNA) on the ER membrane to yield an active transcription factor (XBP1s), leading to the alleviation of the stress. The nascent peptide encoded by XBP1u mRNA drags the mRNA-ribosome- nascent chain (R-RNC) complex to the membrane for efficient cytoplasmic splicing. We found that translation of the XBP1u mRNA was briefly paused to stabilize the R-RNC complex. Mutational analysis of XBP1u revealed an evolutionarily conserved peptide module at the carboxyl terminus that was responsible for the translational pausing and was required for the efficient targeting and splicing of the XBP1u mRNA. Thus, translational pausing may be used for unexpectedly diverse cellular processes in mammalian cells.

Original languageEnglish
Pages (from-to)586-589
Number of pages4
JournalScience
Volume331
Issue number6017
DOIs
Publication statusPublished - Feb 4 2011
Externally publishedYes

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Cell Membrane
Messenger RNA
Endoribonucleases
Peptides
Endoplasmic Reticulum Stress
Protein Biosynthesis
Inositol
Ribosomes
Endoplasmic Reticulum
Transcription Factors
Membranes
Enzymes

All Science Journal Classification (ASJC) codes

  • General

Cite this

Translational pausing ensures membrane targeting and cytoplasmic splicing of XBP1u mRNA. / Yanagitani, Kota; Kimata, Yukio; Kadokura, Hiroshi; Kohno, Kenji.

In: Science, Vol. 331, No. 6017, 04.02.2011, p. 586-589.

Research output: Contribution to journalArticle

Yanagitani, Kota ; Kimata, Yukio ; Kadokura, Hiroshi ; Kohno, Kenji. / Translational pausing ensures membrane targeting and cytoplasmic splicing of XBP1u mRNA. In: Science. 2011 ; Vol. 331, No. 6017. pp. 586-589.
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