Translational regulation of complement protein C2 expression by differential utilization of the 5'-untranslated region of mRNA

Takahiko Horiuchi, K. J. Macon, V. J. Kidd, J. E. Volanakis

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

A recently isolated cDNA for human complement protein C2, C2HL5-3, has an unusually long 5'-untranslated region (UTR) containing four open reading frames upstream of the authentic initiation codon. Here we report that deletion of the 5'-UTR of C2HL5-3 resulted in a 10-fold enhancement in the translational efficiency of C2 in transient eukaryotic cellular assays. Elimination of the open reading frames in the 5'-UTR by site-directed mutagenesis of the initiation codons did not affect C2 synthesis in this assay system, indicating that other structural elements in this region are responsible for translational control. We also show that several C2 mRNAs of varying length in the 5'-UTR, including an alternative C2 mRNA expressing a considerably shorter 5'-UTR, are present in the liver and that the shorter C2 mRNA is the only C2 message found in U937 cells. These results suggest that tissue-specific utilization of alternative transcriptional start sites results in differential translational efficiencies of C2 and may provide insights into the tissue- and stimulus-specific regulation of eukaryotic gene expression.

Original languageEnglish
Pages (from-to)6521-6524
Number of pages4
JournalJournal of Biological Chemistry
Volume265
Issue number12
Publication statusPublished - May 16 1990

Fingerprint

Complement C2
5' Untranslated Regions
Complement System Proteins
Messenger RNA
Initiator Codon
Open Reading Frames
Assays
Tissue
Mutagenesis
U937 Cells
Gene Expression Regulation
Site-Directed Mutagenesis
Gene expression
Liver
Complementary DNA

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Translational regulation of complement protein C2 expression by differential utilization of the 5'-untranslated region of mRNA. / Horiuchi, Takahiko; Macon, K. J.; Kidd, V. J.; Volanakis, J. E.

In: Journal of Biological Chemistry, Vol. 265, No. 12, 16.05.1990, p. 6521-6524.

Research output: Contribution to journalArticle

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