Translational regulation of platelet-derived growth factor B chain (c-sis) mRNA by short open reading frames in the 5'-untranslated region

H. Nikami, T. Kobayashi, T. Tasaki, S. Yoshino, S. Taharaguchi, Etsuro Ono

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Platelet-derived growth factor B chain (PDGF-B/c-SIS), the product of c-sis proto-oncogene, is a potent mitogen and chemoattractant for cells of mesenchymal origin. Expression of PDGF-B/c-SIS is regulated at the translational level, in addition to at the transcriptional level. The 5'-untranslated region (5'-UTR) of PDGF-B/c-sis mRNA is known to inhibit translation of the downstream coding sequences. The 5'-UTR contains putative influential elements, such as GC-rich elements, stem-loop structures and short open reading frames (SORFs). To clarify the inhibition mechanism of PDGF-B/c-sis mRNA translation, effects of three SORFs in the 5'-UTR on the translational regulation were investigated in transient expression assays. Introducing point mutation(s) in the initiation codons of SORFs affected the reporter gene expression in several cell lines (COS-1, U-2, JEG-3). Abrogation of three SORFs resulted in an increase of the reporter gene expression both in β-galactosidase assay and Western blot analysis. These results suggest that SORFs in the 5'-UTR sequences have inhibitory effects on the translation of the downstream coding sequences.

Original languageEnglish
Pages (from-to)166-170
Number of pages5
JournalPlatelets
Volume11
Issue number3
Publication statusPublished - Jun 22 2000
Externally publishedYes

Fingerprint

Proto-Oncogene Proteins c-sis
5' Untranslated Regions
Open Reading Frames
Messenger RNA
Reporter Genes
Galactosidases
Gene Expression
Initiator Codon
Chemotactic Factors
Protein Biosynthesis
Mitogens
Point Mutation
Western Blotting
Cell Line

All Science Journal Classification (ASJC) codes

  • Hematology

Cite this

Translational regulation of platelet-derived growth factor B chain (c-sis) mRNA by short open reading frames in the 5'-untranslated region. / Nikami, H.; Kobayashi, T.; Tasaki, T.; Yoshino, S.; Taharaguchi, S.; Ono, Etsuro.

In: Platelets, Vol. 11, No. 3, 22.06.2000, p. 166-170.

Research output: Contribution to journalArticle

Nikami, H. ; Kobayashi, T. ; Tasaki, T. ; Yoshino, S. ; Taharaguchi, S. ; Ono, Etsuro. / Translational regulation of platelet-derived growth factor B chain (c-sis) mRNA by short open reading frames in the 5'-untranslated region. In: Platelets. 2000 ; Vol. 11, No. 3. pp. 166-170.
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