Translesion replication of benzo[a]pyrene and benzo[c]phenantherene diol epoxide adducts of deoxyadenosine and deoxyguanosine by human DNA polymerase ι

Ekaterina G. Frank, Jane M. Sayer, Heiko Kroth, Eiji Ohashi, Haruo Ohmori, Donald M. Jerina, Roger Woodgate

Research output: Contribution to journalReview article

62 Citations (Scopus)

Abstract

Human DNA polymerase ι (polι) is a Y-family polymerase whose cellular function is presently unknown. Here, we report on the ability of polι to bypass various stereoisomers of benzo[a]pyrene (BaP) diol epoxide (DE) and benzo[c]phenanthrene (BcPh) DE adducts at deoxyadenosine (dA) or deoxyguanosine (dG) bases in four different template sequence contexts in vitro. We find that the BaP DE dG adducts pose a strong block to polι-dependent replication and result in a high frequency of base misincorporations. In contrast, misincorporations opposite BaP DE and BcPh DE dA adducts generally occurred with a frequency ranging between 2 × 10-3 and 6 × 10-4. Although dTMP was inserted efficiently opposite all dA adducts, further extension was relatively poor, with one exception (a cis opened adduct derived from BcPh DE) where up to 58% extension past the lesion was observed. Interestingly, another human Y-family polymerase, polκ, was able to extend dTMP inserted opposite a BaP DE dA adduct. We suggest that polι might therefore participate in the error-free bypass of DE-adducted dA in vivo by predominantly incorporating dTMP opposite the damaged base. In many cases, elongation would, however, require the participation of another polymerase more specialized in extension, such as polκ.

Original languageEnglish
Pages (from-to)5284-5292
Number of pages9
JournalNucleic acids research
Volume30
Issue number23
DOIs
Publication statusPublished - Dec 1 2002

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Deoxyguanosine
Benzo(a)pyrene
Epoxy Compounds
DNA-Directed DNA Polymerase
Stereoisomerism
2'-deoxyadenosine

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Translesion replication of benzo[a]pyrene and benzo[c]phenantherene diol epoxide adducts of deoxyadenosine and deoxyguanosine by human DNA polymerase ι. / Frank, Ekaterina G.; Sayer, Jane M.; Kroth, Heiko; Ohashi, Eiji; Ohmori, Haruo; Jerina, Donald M.; Woodgate, Roger.

In: Nucleic acids research, Vol. 30, No. 23, 01.12.2002, p. 5284-5292.

Research output: Contribution to journalReview article

Frank, Ekaterina G. ; Sayer, Jane M. ; Kroth, Heiko ; Ohashi, Eiji ; Ohmori, Haruo ; Jerina, Donald M. ; Woodgate, Roger. / Translesion replication of benzo[a]pyrene and benzo[c]phenantherene diol epoxide adducts of deoxyadenosine and deoxyguanosine by human DNA polymerase ι. In: Nucleic acids research. 2002 ; Vol. 30, No. 23. pp. 5284-5292.
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