Transluminal gene transfer into brain capillary endothelial cells in vivo with HVJ-liposomes

Chen Jiang, Hirotami Matsuo, Noriko Koyabu, Hisakazu Ohtani, Hidenori Fujimoto, Yoshikazu Yonemitsu, Yasufumi Kaneda, Mikihiko Naito, Takashi Tsuruo, Yasufumi Sawada

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13 Citations (Scopus)

Abstract

Bioactive proteins or peptides cannot be effectively delivered into brain capillary endothelial cells (BCECs) or brain parenchyma. In this study, we selectively transferred Escherichia coli β-galactosidase gene (β-gal) as a model gene into BCECs by using the hemagglutination virus of Japan (HVJ)-liposomes. HVJ-liposomes encapsulating a β-gal plasmid were used to transfect MBEC4 cells in vitro, and were administrated via the internal carotid artery to rat in vivo. Success of the procedure was confirmed by the detection of 116 kDa β-gal protein in transfected MBEC4 cells and in brain capillaries isolated from transfected rats, by Western blot analysis and histological staining. The enzymatic activities of β-galactosidase were 5- to 10-fold and 20-fold higher than when β-gal-containing liposomes without fusogenic activity (uncoated liposomes) or plasmid alone were employed in vitro and in vivo, respectively. Thus, HVJ-liposomes were demonstrated to be a useful vector to transfer a foreign gene into the brain capillary endothelium in vivo via the transluminal route.

Original languageEnglish
Pages (from-to)345-352
Number of pages8
JournalJournal of Drug Targeting
Volume10
Issue number4
DOIs
Publication statusPublished - 2002

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science

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    Jiang, C., Matsuo, H., Koyabu, N., Ohtani, H., Fujimoto, H., Yonemitsu, Y., Kaneda, Y., Naito, M., Tsuruo, T., & Sawada, Y. (2002). Transluminal gene transfer into brain capillary endothelial cells in vivo with HVJ-liposomes. Journal of Drug Targeting, 10(4), 345-352. https://doi.org/10.1080/10611860290031822