Treatment of poplar callus with ferulic and sinapic acids II: Effects on related monolignol biosynthetic enzyme activities

Katsuyoshi Hamada, Yuji Tsutsumi, Tomoaki Nishida

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

In a previous study we reported that administered ferulic acid (FA) was incorporated into guaiacyl and syringyl lignin in poplar callus (Populus alba L.) but administered sinapic acid (SA) was not. It is possible that SA may induce or enhance the biosynthesis of syringyl lignin. In this study, enzyme activities in lignin biosynthesis were assayed in FA- or SA-treated callus. The activity of 4-coumarate:coenzyme A ligase (4CL) toward FA in FA-treated callus was not affected, and its activity toward caffeic and sinapic acids was quite low. In addition, phenylalanine ammonia-lyase (PAL) activity. cinnamate:4-hydroxylase (C4H) transcription in the upstream steps of lignin biosynthesis, also was not affected. 4CL from SA-treated callus exhibited no detectable activity toward SA, suggesting that SA is not a precursor for syringyl lignin. However, all upstream steps of lignin biosynthesis (PAL activity, C4H transcription, 4CL activity toward FA) exhibited increased activity. These results suggest that SA may induce or enhance enzyme activities involved in syringyl lignin biosynthesis in poplar callus.

Original languageEnglish
Pages (from-to)366-370
Number of pages5
JournalJournal of Wood Science
Volume49
Issue number4
DOIs
Publication statusPublished - Oct 31 2003

Fingerprint

ferulic acid
sinapic acid
Enzyme activity
Lignin
enzyme activity
callus
lignin
Biosynthesis
Acids
acid
Coenzyme A Ligases
biosynthesis
Trans-Cinnamate 4-Monooxygenase
coenzyme A
ligases
trans-cinnamate 4-monooxygenase
Phenylalanine Ammonia-Lyase
Coenzymes
phenylalanine ammonia-lyase
Transcription

All Science Journal Classification (ASJC) codes

  • Biomaterials

Cite this

Treatment of poplar callus with ferulic and sinapic acids II : Effects on related monolignol biosynthetic enzyme activities. / Hamada, Katsuyoshi; Tsutsumi, Yuji; Nishida, Tomoaki.

In: Journal of Wood Science, Vol. 49, No. 4, 31.10.2003, p. 366-370.

Research output: Contribution to journalArticle

@article{d5e7efa5863740a2a13fa719cbfd513e,
title = "Treatment of poplar callus with ferulic and sinapic acids II: Effects on related monolignol biosynthetic enzyme activities",
abstract = "In a previous study we reported that administered ferulic acid (FA) was incorporated into guaiacyl and syringyl lignin in poplar callus (Populus alba L.) but administered sinapic acid (SA) was not. It is possible that SA may induce or enhance the biosynthesis of syringyl lignin. In this study, enzyme activities in lignin biosynthesis were assayed in FA- or SA-treated callus. The activity of 4-coumarate:coenzyme A ligase (4CL) toward FA in FA-treated callus was not affected, and its activity toward caffeic and sinapic acids was quite low. In addition, phenylalanine ammonia-lyase (PAL) activity. cinnamate:4-hydroxylase (C4H) transcription in the upstream steps of lignin biosynthesis, also was not affected. 4CL from SA-treated callus exhibited no detectable activity toward SA, suggesting that SA is not a precursor for syringyl lignin. However, all upstream steps of lignin biosynthesis (PAL activity, C4H transcription, 4CL activity toward FA) exhibited increased activity. These results suggest that SA may induce or enhance enzyme activities involved in syringyl lignin biosynthesis in poplar callus.",
author = "Katsuyoshi Hamada and Yuji Tsutsumi and Tomoaki Nishida",
year = "2003",
month = "10",
day = "31",
doi = "10.1007/s10086-002-0475-9",
language = "English",
volume = "49",
pages = "366--370",
journal = "Journal of Wood Science",
issn = "1435-0211",
publisher = "Springer Heidelberg",
number = "4",

}

TY - JOUR

T1 - Treatment of poplar callus with ferulic and sinapic acids II

T2 - Effects on related monolignol biosynthetic enzyme activities

AU - Hamada, Katsuyoshi

AU - Tsutsumi, Yuji

AU - Nishida, Tomoaki

PY - 2003/10/31

Y1 - 2003/10/31

N2 - In a previous study we reported that administered ferulic acid (FA) was incorporated into guaiacyl and syringyl lignin in poplar callus (Populus alba L.) but administered sinapic acid (SA) was not. It is possible that SA may induce or enhance the biosynthesis of syringyl lignin. In this study, enzyme activities in lignin biosynthesis were assayed in FA- or SA-treated callus. The activity of 4-coumarate:coenzyme A ligase (4CL) toward FA in FA-treated callus was not affected, and its activity toward caffeic and sinapic acids was quite low. In addition, phenylalanine ammonia-lyase (PAL) activity. cinnamate:4-hydroxylase (C4H) transcription in the upstream steps of lignin biosynthesis, also was not affected. 4CL from SA-treated callus exhibited no detectable activity toward SA, suggesting that SA is not a precursor for syringyl lignin. However, all upstream steps of lignin biosynthesis (PAL activity, C4H transcription, 4CL activity toward FA) exhibited increased activity. These results suggest that SA may induce or enhance enzyme activities involved in syringyl lignin biosynthesis in poplar callus.

AB - In a previous study we reported that administered ferulic acid (FA) was incorporated into guaiacyl and syringyl lignin in poplar callus (Populus alba L.) but administered sinapic acid (SA) was not. It is possible that SA may induce or enhance the biosynthesis of syringyl lignin. In this study, enzyme activities in lignin biosynthesis were assayed in FA- or SA-treated callus. The activity of 4-coumarate:coenzyme A ligase (4CL) toward FA in FA-treated callus was not affected, and its activity toward caffeic and sinapic acids was quite low. In addition, phenylalanine ammonia-lyase (PAL) activity. cinnamate:4-hydroxylase (C4H) transcription in the upstream steps of lignin biosynthesis, also was not affected. 4CL from SA-treated callus exhibited no detectable activity toward SA, suggesting that SA is not a precursor for syringyl lignin. However, all upstream steps of lignin biosynthesis (PAL activity, C4H transcription, 4CL activity toward FA) exhibited increased activity. These results suggest that SA may induce or enhance enzyme activities involved in syringyl lignin biosynthesis in poplar callus.

UR - http://www.scopus.com/inward/record.url?scp=0142103234&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0142103234&partnerID=8YFLogxK

U2 - 10.1007/s10086-002-0475-9

DO - 10.1007/s10086-002-0475-9

M3 - Article

AN - SCOPUS:0142103234

VL - 49

SP - 366

EP - 370

JO - Journal of Wood Science

JF - Journal of Wood Science

SN - 1435-0211

IS - 4

ER -