Trehalose, a naturally occurring osmolyte, appears to be one of the most effective protectants for enzymes under various stress conditions while urea, a classical denaturant, destabilizes the activity, function, and alters the native structure of proteins. Herein, we have characterized the counteracting effects of trehalose on the deleterious effect of urea on α-chymotrypsin (CT) through the calorimetric data (transition temperature (Tm), enthalpy change (ΔH), heat capacity change (ΔCp) and Gibbs free energy of unfolding (ΔGu) by using differential scanning calorimeter (DSC) and circular dichroism (CD) techniques, respectively, at a 1:2 ratio of trehalose and urea, as well as various urea concentration (up to 6M) in the presence of 1M trehalose. Our parallel experimental results explicitly elucidate that trehalose strongly offset the deleterious actions of urea on CT at 1:2 molar ratio of trehalose and urea, however, trehalose (1M) some how failed to counteract the perturbation effects of urea (3-6M) on CT.
|Number of pages||6|
|Journal||International Journal of Biological Macromolecules|
|Publication status||Published - Nov 1 2010|
All Science Journal Classification (ASJC) codes
- Structural Biology
- Molecular Biology
- Economics and Econometrics