Tricellulin constitutes a novel barrier at tricellular contacts of epithelial cells

Junichi Ikenouchi, Mikio Furuse, Kyoko Furuse, Hiroyuki Sasaki, Sachiko Tsukita, Shoichiro Tsukita

Research output: Contribution to journalArticle

466 Citations (Scopus)

Abstract

For epithelia to function as barriers, the intercellular space must be sealed. Sealing two adjacent cells at bicellular tight junctions (bTJs) is well described with the discovery of the claudins. Yet, there are still barrier weak points at tricellular contacts, where three cells join together. In this study, we identify tricellulin, the first integral membrane protein that is concentrated at the vertically oriented TJ strands of tricellular contacts. When tricellulin expression was suppressed with RNA interference, the epithelial barrier was compromised, and tricellular contacts and bTJs were disorganized. These findings indicate the critical function of tricellulin for formation of the epithelial barrier.

Original languageEnglish
Pages (from-to)939-945
Number of pages7
JournalJournal of Cell Biology
Volume171
Issue number6
DOIs
Publication statusPublished - Dec 19 2005

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MARVEL Domain Containing 2 Protein
Tight Junctions
Epithelial Cells
Claudins
Extracellular Space
RNA Interference
Membrane Proteins
Epithelium

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

Tricellulin constitutes a novel barrier at tricellular contacts of epithelial cells. / Ikenouchi, Junichi; Furuse, Mikio; Furuse, Kyoko; Sasaki, Hiroyuki; Tsukita, Sachiko; Tsukita, Shoichiro.

In: Journal of Cell Biology, Vol. 171, No. 6, 19.12.2005, p. 939-945.

Research output: Contribution to journalArticle

Ikenouchi, Junichi ; Furuse, Mikio ; Furuse, Kyoko ; Sasaki, Hiroyuki ; Tsukita, Sachiko ; Tsukita, Shoichiro. / Tricellulin constitutes a novel barrier at tricellular contacts of epithelial cells. In: Journal of Cell Biology. 2005 ; Vol. 171, No. 6. pp. 939-945.
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