In this study, we revealed that a trifluoroleucine-resistant mutant of Saccharomyces cerevisiae also exhibits pleiotropic drug resistance. DNA microarray analysis of wild-type strain vs. a trifluoroleucine-resistant mutant showed that the PDR5, PDR10, PDR15, and YOR1 genes, encoding the ABC transporter, had high levels of expression. By contrast, the 9 transcriptional factor genes of the ABC transporter, such as PDR1 and PDR3, and the repressor of drug resistance, RDR1, were not up-regulated. When the nucleotide sequences of PDR1 and PDR3 of the wild-type strain and trifluoroleucine-resistant mutant were compared, the sequences of both genes in each strain were identical.
All Science Journal Classification (ASJC) codes
- Applied Microbiology and Biotechnology