Three previously undescribed triterpene saponins, and four known ones, were isolated from the EtOH/H2O extract of the roots of Bupleurum spinosum. Their structures were characterized using spectroscopic techniques including 1D and 2D NMR (1H, 13C, COSY, TOCSY, ROESY, HSQC, and HMBC) experiments and mass spectrometry as 3-O-β-D-glucopyranosyl-(1→6)-[β-D-glucopyranosyl-(1→2)]-[α-L-rhamnopyranosyl-(1→4)]-β-D-glucopyranosyl-3β,16β,23,28-tetrahydroxyolean-12-ene, 3-O-β-D-glucopyranosyl-(1→6)-[β-D-glucopyranosyl-(1→2)]-[α-L-rhamnopyranosyl-(1→4)]-β-D-glucopyranosyl-3β,16β,28-trihydroxy-23-oxoolean-12-ene, 3-O-β-D-glucopyranosyl-(1→6)-[α-L-rhamnopyranosyl(1→4)]-β-D-glucopyranosyl-3β,16β,28-trihydroxyolean-12-ene-23-oic-acid. The chemotaxonomic significance is underlined.
All Science Journal Classification (ASJC) codes
- Agronomy and Crop Science
- Plant Science