Troglitazone inhibits the capacitative Ca2+ entry in endothelial cells

Junya Kawasaki, Katsuya Hirano, Mayumi Hirano, Junji Nishimura, Masatoshi Fujishima, Hideo Kanaide

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

To investigate the effects of troglitazone on the capacitative Ca2+ entry, we monitored changes in cytosolic Ca2+ concentrations ([Ca2+](i)) induced by thapsigargin in fura-2-loaded porcine endothelial cells in situ and in primary culture. In aortic valve endothelial cells in situ, thapsigargin induced sustained elevation of [Ca2+](i). Both troglitazone and SKF 96365 inhibited the steady state increase in [Ca2+](i) in a concentration-dependent manner. At 30 μM, troglitazone and SKF 96365 inhibited the [Ca2+](i) elevation to 19.4 ± 3.6% and 43.9 ± 4.5%, respectively. In aortic endothelial cells in primary culture, both troglitazone (10 μM) and SKF 96365 (100 μM) completely inhibited the thapsigargin-induced [Ca2+](i) increase. The EC50 value of troglitazone (1.4 ± 0.1 μM) was lower than that of SKF 96365 (10.0 ± 3.3 μM). We suggest that troglitazone would be a useful tool to investigate the capacitative Ca2+ entry.

Original languageEnglish
Pages (from-to)111-120
Number of pages10
JournalEuropean Journal of Pharmacology
Volume373
Issue number1
DOIs
Publication statusPublished - May 28 1999
Externally publishedYes

Fingerprint

troglitazone
1-(2-(3-(4-methoxyphenyl)propoxy)-4-methoxyphenylethyl)-1H-imidazole
Endothelial Cells
Thapsigargin
Fura-2
Aortic Valve
Swine

All Science Journal Classification (ASJC) codes

  • Pharmacology

Cite this

Troglitazone inhibits the capacitative Ca2+ entry in endothelial cells. / Kawasaki, Junya; Hirano, Katsuya; Hirano, Mayumi; Nishimura, Junji; Fujishima, Masatoshi; Kanaide, Hideo.

In: European Journal of Pharmacology, Vol. 373, No. 1, 28.05.1999, p. 111-120.

Research output: Contribution to journalArticle

Kawasaki, Junya ; Hirano, Katsuya ; Hirano, Mayumi ; Nishimura, Junji ; Fujishima, Masatoshi ; Kanaide, Hideo. / Troglitazone inhibits the capacitative Ca2+ entry in endothelial cells. In: European Journal of Pharmacology. 1999 ; Vol. 373, No. 1. pp. 111-120.
@article{e8e5e1706c67457386ba501ffd7d750c,
title = "Troglitazone inhibits the capacitative Ca2+ entry in endothelial cells",
abstract = "To investigate the effects of troglitazone on the capacitative Ca2+ entry, we monitored changes in cytosolic Ca2+ concentrations ([Ca2+](i)) induced by thapsigargin in fura-2-loaded porcine endothelial cells in situ and in primary culture. In aortic valve endothelial cells in situ, thapsigargin induced sustained elevation of [Ca2+](i). Both troglitazone and SKF 96365 inhibited the steady state increase in [Ca2+](i) in a concentration-dependent manner. At 30 μM, troglitazone and SKF 96365 inhibited the [Ca2+](i) elevation to 19.4 ± 3.6{\%} and 43.9 ± 4.5{\%}, respectively. In aortic endothelial cells in primary culture, both troglitazone (10 μM) and SKF 96365 (100 μM) completely inhibited the thapsigargin-induced [Ca2+](i) increase. The EC50 value of troglitazone (1.4 ± 0.1 μM) was lower than that of SKF 96365 (10.0 ± 3.3 μM). We suggest that troglitazone would be a useful tool to investigate the capacitative Ca2+ entry.",
author = "Junya Kawasaki and Katsuya Hirano and Mayumi Hirano and Junji Nishimura and Masatoshi Fujishima and Hideo Kanaide",
year = "1999",
month = "5",
day = "28",
doi = "10.1016/S0014-2999(99)00257-5",
language = "English",
volume = "373",
pages = "111--120",
journal = "European Journal of Pharmacology",
issn = "0014-2999",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Troglitazone inhibits the capacitative Ca2+ entry in endothelial cells

AU - Kawasaki, Junya

AU - Hirano, Katsuya

AU - Hirano, Mayumi

AU - Nishimura, Junji

AU - Fujishima, Masatoshi

AU - Kanaide, Hideo

PY - 1999/5/28

Y1 - 1999/5/28

N2 - To investigate the effects of troglitazone on the capacitative Ca2+ entry, we monitored changes in cytosolic Ca2+ concentrations ([Ca2+](i)) induced by thapsigargin in fura-2-loaded porcine endothelial cells in situ and in primary culture. In aortic valve endothelial cells in situ, thapsigargin induced sustained elevation of [Ca2+](i). Both troglitazone and SKF 96365 inhibited the steady state increase in [Ca2+](i) in a concentration-dependent manner. At 30 μM, troglitazone and SKF 96365 inhibited the [Ca2+](i) elevation to 19.4 ± 3.6% and 43.9 ± 4.5%, respectively. In aortic endothelial cells in primary culture, both troglitazone (10 μM) and SKF 96365 (100 μM) completely inhibited the thapsigargin-induced [Ca2+](i) increase. The EC50 value of troglitazone (1.4 ± 0.1 μM) was lower than that of SKF 96365 (10.0 ± 3.3 μM). We suggest that troglitazone would be a useful tool to investigate the capacitative Ca2+ entry.

AB - To investigate the effects of troglitazone on the capacitative Ca2+ entry, we monitored changes in cytosolic Ca2+ concentrations ([Ca2+](i)) induced by thapsigargin in fura-2-loaded porcine endothelial cells in situ and in primary culture. In aortic valve endothelial cells in situ, thapsigargin induced sustained elevation of [Ca2+](i). Both troglitazone and SKF 96365 inhibited the steady state increase in [Ca2+](i) in a concentration-dependent manner. At 30 μM, troglitazone and SKF 96365 inhibited the [Ca2+](i) elevation to 19.4 ± 3.6% and 43.9 ± 4.5%, respectively. In aortic endothelial cells in primary culture, both troglitazone (10 μM) and SKF 96365 (100 μM) completely inhibited the thapsigargin-induced [Ca2+](i) increase. The EC50 value of troglitazone (1.4 ± 0.1 μM) was lower than that of SKF 96365 (10.0 ± 3.3 μM). We suggest that troglitazone would be a useful tool to investigate the capacitative Ca2+ entry.

UR - http://www.scopus.com/inward/record.url?scp=0033053093&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033053093&partnerID=8YFLogxK

U2 - 10.1016/S0014-2999(99)00257-5

DO - 10.1016/S0014-2999(99)00257-5

M3 - Article

VL - 373

SP - 111

EP - 120

JO - European Journal of Pharmacology

JF - European Journal of Pharmacology

SN - 0014-2999

IS - 1

ER -