Troponin I inhibitory peptide suppresses the force generation in smooth muscle by directly interfering with cross-bridge formation

Masaru Watanabe, Yasumasa Yoshino, Sachio Morimoto

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

To explore possible mechanisms involving the thin filament-linked regulation of contraction in living smooth muscles, we studied the effects of a synthetic peptide of rabbit cardiac troponin I [residues 136-147] (TnIp), which is a minimal sequence required to inhibit striated muscle acto-tropomyosin-myosin ATPase activity, on the mechanical properties of β-escin skinned preparations of taenia caeci from guinea pig. TnIp reversibly suppressed the Ca2+-activated force without significant effects on the Ca2+ sensitivity and on the phosphorylation level of myosin regulatory light chain (MLC20). TnIp also reversibly suppressed the Ca2+/calmodulin-independent contraction induced by 30mM Mg2+. An analogue of TnIp, which lost inhibiting action on acto-tropomyosin-myosin ATPase activity, affected neither Ca2+-activated nor 30mM Mg2+-induced contraction. These results indicate that TnIp suppresses the force generation in smooth muscle by directly interfering with cross-bridge formation rather than inhibiting the Ca2+/calmodulin-dependent thick and thin filament activating processes.

Original languageEnglish
Pages (from-to)236-240
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume307
Issue number2
DOIs
Publication statusPublished - Jul 25 2003

Fingerprint

pioglitazone
Tropomyosin
Troponin I
Calmodulin
Myosins
Smooth Muscle
Muscle
Escin
Taenia
Myosin Light Chains
Peptides
Striated Muscle
Guinea Pigs
Phosphorylation
Rabbits
Mechanical properties

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Troponin I inhibitory peptide suppresses the force generation in smooth muscle by directly interfering with cross-bridge formation. / Watanabe, Masaru; Yoshino, Yasumasa; Morimoto, Sachio.

In: Biochemical and Biophysical Research Communications, Vol. 307, No. 2, 25.07.2003, p. 236-240.

Research output: Contribution to journalArticle

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