TRPV4 associates environmental temperature and sex determination in the American alligator

Ryohei Yatsu, Shinichi Miyagawa, Satomi Kohno, Shigeru Saito, Russell H. Lowers, Yukiko Ogino, Naomi Fukuta, Yoshinao Katsu, Yasuhiko Ohta, Makoto Tominaga, Louis J. Guillette, Taisen Iguchi

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Abstract

Temperature-dependent sex determination (TSD), commonly found among reptiles, is a sex determination mode in which the incubation temperature during a critical temperature sensitive period (TSP) determines sexual fate of the individual rather than the individualâ €™ s genotypic background. In the American alligator (Alligator mississippiensis), eggs incubated during the TSP at 33 °C (male producing temperature: MPT) yields male offspring, whereas incubation temperatures below 30 °C (female producing temperature: FPT) lead to female offspring. However, many of the details of the underlying molecular mechanism remains elusive, and the molecular link between environmental temperature and sex determination pathway is yet to be elucidated. Here we show the alligator TRPV4 ortholog (AmTRPV4) to be activated at temperatures proximate to the TSD-related temperature in alligators, and using pharmacological exposure, we show that AmTRPV4 channel activity affects gene expression patterns associated with male differentiation. This is the first experimental demonstration of a link between a well-described thermo-sensory mechanism, TRPV4 channel, and its potential role in regulation of TSD in vertebrates, shedding unique new light on the elusive TSD molecular mechanism.

Original languageEnglish
Article number18581
JournalScientific reports
Volume5
DOIs
Publication statusPublished - Dec 18 2015

All Science Journal Classification (ASJC) codes

  • General

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    Yatsu, R., Miyagawa, S., Kohno, S., Saito, S., Lowers, R. H., Ogino, Y., Fukuta, N., Katsu, Y., Ohta, Y., Tominaga, M., Guillette, L. J., & Iguchi, T. (2015). TRPV4 associates environmental temperature and sex determination in the American alligator. Scientific reports, 5, [18581]. https://doi.org/10.1038/srep18581