Tryptophan-19 of β-lactoglobulin, the only residue completely conserved in the lipocalin superfamily, is not essential for binding retinol, but relevant to stabilizing bound retinol and maintaining its structure

Yoshinori Katakura, Mamoru Totsuka, Akio Ametani, Shuichi Kaminogawa

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Abstract

Residue 19 of tryptophan in bovine β-lactoglobulin (β-LG) is the only invariant residue throughout the lipocalin superfamily having two characteristic features: binding ability for small hydrophobic molecules and the unique β-barrel three-dimensional structure. In this study, we investigated whether this strictly conserved Trp-19 of β-LG would be indispensable for its structure and function such as maintaining the molecular structure and biological activity of β-LG. Spectroscopic and enzymatic oxidation experiments on retinol bound to W19Y, in which Tyr was substituted for Trp-19, showed that Trp-19 was not critical for this binding, but was important for stably maintaining the environment surrounding retinol and the bound retinol. An analysis, using four anti-β-LG monoclonal antibodies as probes, revealed a structural change in region 20-29, but not in the reverse region of Trp-19. A guanidine hydrochloride-induced unfolding study showed that the conformational stability of W19Y was greatly reduced by 6.9 kcal/mol compared to that of wild-type β-LG. These facts indicated that Trp-19 is one of the important residues in correctly maintaining the local structure of β-LG and stably retaining its overall structure, thereby conserving the bound retinol molecule.

Original languageEnglish
Pages (from-to)58-67
Number of pages10
JournalBiochimica et Biophysica Acta (BBA)/Protein Structure and Molecular
Volume1207
Issue number1
DOIs
Publication statusPublished - Jul 20 1994
Externally publishedYes

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Lipocalins
Lactoglobulins
Vitamin A
Tryptophan
Molecules
Guanidine
Bioactivity
Molecular Structure
Molecular structure
Monoclonal Antibodies
Oxidation
Experiments

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Structural Biology

Cite this

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title = "Tryptophan-19 of β-lactoglobulin, the only residue completely conserved in the lipocalin superfamily, is not essential for binding retinol, but relevant to stabilizing bound retinol and maintaining its structure",
abstract = "Residue 19 of tryptophan in bovine β-lactoglobulin (β-LG) is the only invariant residue throughout the lipocalin superfamily having two characteristic features: binding ability for small hydrophobic molecules and the unique β-barrel three-dimensional structure. In this study, we investigated whether this strictly conserved Trp-19 of β-LG would be indispensable for its structure and function such as maintaining the molecular structure and biological activity of β-LG. Spectroscopic and enzymatic oxidation experiments on retinol bound to W19Y, in which Tyr was substituted for Trp-19, showed that Trp-19 was not critical for this binding, but was important for stably maintaining the environment surrounding retinol and the bound retinol. An analysis, using four anti-β-LG monoclonal antibodies as probes, revealed a structural change in region 20-29, but not in the reverse region of Trp-19. A guanidine hydrochloride-induced unfolding study showed that the conformational stability of W19Y was greatly reduced by 6.9 kcal/mol compared to that of wild-type β-LG. These facts indicated that Trp-19 is one of the important residues in correctly maintaining the local structure of β-LG and stably retaining its overall structure, thereby conserving the bound retinol molecule.",
author = "Yoshinori Katakura and Mamoru Totsuka and Akio Ametani and Shuichi Kaminogawa",
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T1 - Tryptophan-19 of β-lactoglobulin, the only residue completely conserved in the lipocalin superfamily, is not essential for binding retinol, but relevant to stabilizing bound retinol and maintaining its structure

AU - Katakura, Yoshinori

AU - Totsuka, Mamoru

AU - Ametani, Akio

AU - Kaminogawa, Shuichi

PY - 1994/7/20

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N2 - Residue 19 of tryptophan in bovine β-lactoglobulin (β-LG) is the only invariant residue throughout the lipocalin superfamily having two characteristic features: binding ability for small hydrophobic molecules and the unique β-barrel three-dimensional structure. In this study, we investigated whether this strictly conserved Trp-19 of β-LG would be indispensable for its structure and function such as maintaining the molecular structure and biological activity of β-LG. Spectroscopic and enzymatic oxidation experiments on retinol bound to W19Y, in which Tyr was substituted for Trp-19, showed that Trp-19 was not critical for this binding, but was important for stably maintaining the environment surrounding retinol and the bound retinol. An analysis, using four anti-β-LG monoclonal antibodies as probes, revealed a structural change in region 20-29, but not in the reverse region of Trp-19. A guanidine hydrochloride-induced unfolding study showed that the conformational stability of W19Y was greatly reduced by 6.9 kcal/mol compared to that of wild-type β-LG. These facts indicated that Trp-19 is one of the important residues in correctly maintaining the local structure of β-LG and stably retaining its overall structure, thereby conserving the bound retinol molecule.

AB - Residue 19 of tryptophan in bovine β-lactoglobulin (β-LG) is the only invariant residue throughout the lipocalin superfamily having two characteristic features: binding ability for small hydrophobic molecules and the unique β-barrel three-dimensional structure. In this study, we investigated whether this strictly conserved Trp-19 of β-LG would be indispensable for its structure and function such as maintaining the molecular structure and biological activity of β-LG. Spectroscopic and enzymatic oxidation experiments on retinol bound to W19Y, in which Tyr was substituted for Trp-19, showed that Trp-19 was not critical for this binding, but was important for stably maintaining the environment surrounding retinol and the bound retinol. An analysis, using four anti-β-LG monoclonal antibodies as probes, revealed a structural change in region 20-29, but not in the reverse region of Trp-19. A guanidine hydrochloride-induced unfolding study showed that the conformational stability of W19Y was greatly reduced by 6.9 kcal/mol compared to that of wild-type β-LG. These facts indicated that Trp-19 is one of the important residues in correctly maintaining the local structure of β-LG and stably retaining its overall structure, thereby conserving the bound retinol molecule.

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