Tsix RNA and the germline factor, PRDM14, link X reactivation and stem cell reprogramming

Bernhard Payer, Michael Rosenberg, Masashi Yamaji, Yukihiro Yabuta, Michiyo Koyanagi-Aoi, Katsuhiko Hayashi, Shinya Yamanaka, Mitinori Saitou, Jeannie T. Lee

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Transitions between pluripotent and differentiated states are marked by dramatic epigenetic changes. Cellular differentiation is tightly linked to X chromosome inactivation (XCI), whereas reprogramming to induced pluripotent stem cells (iPSCs) is associated with X chromosome reactivation (XCR). XCR reverses the silent state of the inactive X, occurring in mouse blastocysts and germ cells. In spite of its importance, little is known about underlying mechanisms. Here, we examine the role of the long noncoding Tsix RNA and the germline factor, PRDM14. In blastocysts, XCR is perturbed by mutation of either Tsix or Prdm14. In iPSCs, XCR is disrupted only by PRDM14 deficiency, which also affects iPSC derivation and maintenance. We show that Tsix and PRDM14 directly link XCR to pluripotency: first, PRDM14 represses Rnf12 by recruiting polycomb repressive complex 2; second, Tsix enables PRDM14 to bind Xist. Thus, our study provides functional and mechanistic links between cellular and X chromosome reprogramming.

Original languageEnglish
Pages (from-to)805-818
Number of pages14
JournalMolecular Cell
Volume52
Issue number6
DOIs
Publication statusPublished - Dec 26 2013
Externally publishedYes

Fingerprint

X Chromosome
Stem Cells
RNA
Induced Pluripotent Stem Cells
Blastocyst
Polycomb Repressive Complex 2
Long Noncoding RNA
X Chromosome Inactivation
Epigenomics
Germ Cells
Cellular Reprogramming
Maintenance
Mutation

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Payer, B., Rosenberg, M., Yamaji, M., Yabuta, Y., Koyanagi-Aoi, M., Hayashi, K., ... Lee, J. T. (2013). Tsix RNA and the germline factor, PRDM14, link X reactivation and stem cell reprogramming. Molecular Cell, 52(6), 805-818. https://doi.org/10.1016/j.molcel.2013.10.023

Tsix RNA and the germline factor, PRDM14, link X reactivation and stem cell reprogramming. / Payer, Bernhard; Rosenberg, Michael; Yamaji, Masashi; Yabuta, Yukihiro; Koyanagi-Aoi, Michiyo; Hayashi, Katsuhiko; Yamanaka, Shinya; Saitou, Mitinori; Lee, Jeannie T.

In: Molecular Cell, Vol. 52, No. 6, 26.12.2013, p. 805-818.

Research output: Contribution to journalArticle

Payer, B, Rosenberg, M, Yamaji, M, Yabuta, Y, Koyanagi-Aoi, M, Hayashi, K, Yamanaka, S, Saitou, M & Lee, JT 2013, 'Tsix RNA and the germline factor, PRDM14, link X reactivation and stem cell reprogramming', Molecular Cell, vol. 52, no. 6, pp. 805-818. https://doi.org/10.1016/j.molcel.2013.10.023
Payer, Bernhard ; Rosenberg, Michael ; Yamaji, Masashi ; Yabuta, Yukihiro ; Koyanagi-Aoi, Michiyo ; Hayashi, Katsuhiko ; Yamanaka, Shinya ; Saitou, Mitinori ; Lee, Jeannie T. / Tsix RNA and the germline factor, PRDM14, link X reactivation and stem cell reprogramming. In: Molecular Cell. 2013 ; Vol. 52, No. 6. pp. 805-818.
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