Tsukushi expression is dependent on Notch signaling and oscillated in the presomitic mesoderm during chick somitogenesis

Uzzal Kumar Acharjee, Ryu Gejima, M. Felemban Athary Abdulhaleem, M. Asrafuzzaman Riyadh, Hideaki Tanaka, Kunimasa Ohta

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

During somitogenesis, segmentation of the body axis occurs by epithelial somites budding off from the rostral end of the unsegmented presomitic mesoderm (PSM), and its molecular regulation is achieved by a molecular oscillator and signaling molecules. Tsukushi (TSK) is a unique secreted protein and involved in diverse biological cascades in vertebrate embryos by modulating several signaling pathways at the extracellular region. However, the involvement of TSK in somitogenesis remains unknown. In this study, we investigated the detailed expression patterns of TSK at different developmental stages of a chick embryo. Chick-TSK (C-TSK) is expressed in the PSM and shows an oscillation pattern with three phases. The oscillation pattern of C-TSK in the PSM is similar to that of c-Notch1 and c-hairy1, but not to c-Delta1. Our in vitro data showed that Notch signaling is necessary for the normal expression of C-TSK and that expression of C-TSK is an intrinsic property of the anterior PSM. These data suggest that TSK plays a role in chick somitogenesis.

Original languageEnglish
Pages (from-to)625-630
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume465
Issue number3
DOIs
Publication statusPublished - Sep 25 2015
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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