Abstract
We synthesized polymeric gene carriers consisting of poly-L-lysine (PLL) main chain modified both with substrate peptide for protein kinase C (PKC) and alkanethiol (pentadecanethiol). Due to the grafted substrate peptide, the polyplex prepared from these carriers is expected to show gene expression triggered by the phosphorylation of the peptide by intracellular PKC. The modified alkanethiol on the main chain stabilized the polyplex both via disulfide crosslinking and hydrophobic interaction. The polyplex found to show gene expression in vitro when the alkanethiol content in the main chain was enough low (4-mol%-modification of PLLs ε-amine group) to minimize cytotoxic effect. Even though the content of alkanethiol is low, the polyplex had significant stability in a model serum solution and showed longer blood circulation in vivo. The polyplex clearly accumulated in tumor after intravenous injection.
| Original language | English |
|---|---|
| Pages (from-to) | 657-668 |
| Number of pages | 12 |
| Journal | Journal of Biomaterials Science, Polymer Edition |
| Volume | 26 |
| Issue number | 11 |
| DOIs | |
| Publication status | Published - Jul 24 2015 |
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All Science Journal Classification (ASJC) codes
- Biophysics
- Bioengineering
- Biomaterials
- Biomedical Engineering
Cite this
Tumor accumulation of protein kinase-responsive gene carrier/DNA polyplex stabilized by alkanethiol for intravenous injection. / Li, Kai; Sato, Hikari; Kim, Chan Woo; Nakamura, Yuta; Zhao, Guo Xi; Funamoto, Daiki; Nobori, Takanobu; Kishimura, Akihiro; Mori, Takeshi; Katayama, Yoshiki.
In: Journal of Biomaterials Science, Polymer Edition, Vol. 26, No. 11, 24.07.2015, p. 657-668.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Tumor accumulation of protein kinase-responsive gene carrier/DNA polyplex stabilized by alkanethiol for intravenous injection
AU - Li, Kai
AU - Sato, Hikari
AU - Kim, Chan Woo
AU - Nakamura, Yuta
AU - Zhao, Guo Xi
AU - Funamoto, Daiki
AU - Nobori, Takanobu
AU - Kishimura, Akihiro
AU - Mori, Takeshi
AU - Katayama, Yoshiki
PY - 2015/7/24
Y1 - 2015/7/24
N2 - We synthesized polymeric gene carriers consisting of poly-L-lysine (PLL) main chain modified both with substrate peptide for protein kinase C (PKC) and alkanethiol (pentadecanethiol). Due to the grafted substrate peptide, the polyplex prepared from these carriers is expected to show gene expression triggered by the phosphorylation of the peptide by intracellular PKC. The modified alkanethiol on the main chain stabilized the polyplex both via disulfide crosslinking and hydrophobic interaction. The polyplex found to show gene expression in vitro when the alkanethiol content in the main chain was enough low (4-mol%-modification of PLLs ε-amine group) to minimize cytotoxic effect. Even though the content of alkanethiol is low, the polyplex had significant stability in a model serum solution and showed longer blood circulation in vivo. The polyplex clearly accumulated in tumor after intravenous injection.
AB - We synthesized polymeric gene carriers consisting of poly-L-lysine (PLL) main chain modified both with substrate peptide for protein kinase C (PKC) and alkanethiol (pentadecanethiol). Due to the grafted substrate peptide, the polyplex prepared from these carriers is expected to show gene expression triggered by the phosphorylation of the peptide by intracellular PKC. The modified alkanethiol on the main chain stabilized the polyplex both via disulfide crosslinking and hydrophobic interaction. The polyplex found to show gene expression in vitro when the alkanethiol content in the main chain was enough low (4-mol%-modification of PLLs ε-amine group) to minimize cytotoxic effect. Even though the content of alkanethiol is low, the polyplex had significant stability in a model serum solution and showed longer blood circulation in vivo. The polyplex clearly accumulated in tumor after intravenous injection.
UR - http://www.scopus.com/inward/record.url?scp=84934438513&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84934438513&partnerID=8YFLogxK
U2 - 10.1080/09205063.2015.1054922
DO - 10.1080/09205063.2015.1054922
M3 - Article
C2 - 26011738
AN - SCOPUS:84934438513
VL - 26
SP - 657
EP - 668
JO - Journal of Biomaterials Science, Polymer Edition
JF - Journal of Biomaterials Science, Polymer Edition
SN - 0920-5063
IS - 11
ER -