Tumor-homing effect of human mesenchymal stem cells in a TH-MYCN mouse model of neuroblastoma

Koseki Kimura, Tsunao Kishida, Junko Wakao, Tomoko Tanaka, Mayumi Higashi, Shigehisa Fumino, Shigeyoshi Aoi, Taizo Furukawa, Osam Mazda, Tatsuro Tajiri

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Background Human mesenchymal stem cells (hMSCs) are multipotent stem-like cells that are reported to have tumor-suppression effects and migration ability toward damaged tissues or tumors. The aim of this study was to analyze the tumor-homing ability of hMSCs and antitumor potency in a transgenic TH-MYCN mouse model of neuroblastoma (NB). Methods hMSCs (3 × 106) labeled with DiR, a lipophilic near-infrared dye, were intraperitoneally (i.p.) or intravenously (i.v.) administered to the TH-MYCN mice. hMSC in vivo kinetics were assayed using the IVIS® imaging system for 24 h after injection. Immunohistochemistry using human CD90 antibody was also performed to confirm the location of hMSCs in various organs and tumors. Furthermore, the survival curve of TH-MYCN mice treated with hMSCs was compared to a control group administered PBS. Results i.p. hMSCs were recognized in the tumors of TH-MYCN mice by IVIS. hMSCs were also located inside the tumor tissue. Conversely, most of the i.v. hMSCs were captured by the lungs, and migration into the tumors was not noted. There was no significant difference in the survival between the hMSC and control groups. Conclusion The present study suggested that hMSCs may be potential tumor-specific therapeutic delivery vehicles in NB according to their homing potential to tumors.

Original languageEnglish
Pages (from-to)2068-2073
Number of pages6
JournalJournal of Pediatric Surgery
Volume51
Issue number12
DOIs
Publication statusPublished - Dec 1 2016
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Pediatrics, Perinatology, and Child Health
  • Surgery

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