Tumor necrosis factor-α (TNF-α)-induced and interleukin-1β (IL-1β)-induced shedding of TNF receptors from gingival fibroblasts

H. Ohe, S. Takashiba, K. Naruishi, H. H. Chou, H. Yamada, Fusanori Nishimura, H. Arai, Y. Murayama

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Tumor necrosis factor-α (TNF-α) exerts its functions by binding two different receptors (TNFR55 and TNFR75). Both TNFR55 and TNFR75 exist in cell-associated and soluble forms. Soluble TNF receptors (sTNFR), sTNFR55 and sTNFR75, are proteolytically shed upon inflammatory stimuli and then modulate various TNF-α bioactivities. As human gingival fibroblasts (HGF) can be potential targets for TNF-α in inflamed gingiva, we hypothesized that HGF partially modulate the cellular responses to TNF-α by regulating their own TNFR. In this study, the kinetics of expression of cell-associated and soluble forms of both receptors from cultured HGF in response to proinflammatory cytokines TNF-α and interleukin-1β (IL-1β) were investigated in vitro. Both TNF-α and IL-1β upregulated the gene expression of TNFR75 and did not affect that of TNFR55. TNF-α and IL-1β decreased binding of [125I]TNF-α to HGF. Moreover, TNF-α and IL-1β upregulated the release of sTNFR75 from HGF but not that of sTNFR55. These results suggest that HGF under inflammatory conditions may contribute to the inactivation of circulating TNF-α through the preferential induction and shedding of TNFR75.

Original languageEnglish
Pages (from-to)1077-1082
Number of pages6
JournalJournal of Interferon and Cytokine Research
Volume20
Issue number12
DOIs
Publication statusPublished - 2000
Externally publishedYes

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Tumor Necrosis Factor Receptors
Interleukin-1
Tumor Necrosis Factor-alpha
Fibroblasts
Receptors, Tumor Necrosis Factor, Type II
Gingiva
Cytokines
Gene Expression

All Science Journal Classification (ASJC) codes

  • Immunology
  • Virology
  • Cell Biology

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Tumor necrosis factor-α (TNF-α)-induced and interleukin-1β (IL-1β)-induced shedding of TNF receptors from gingival fibroblasts. / Ohe, H.; Takashiba, S.; Naruishi, K.; Chou, H. H.; Yamada, H.; Nishimura, Fusanori; Arai, H.; Murayama, Y.

In: Journal of Interferon and Cytokine Research, Vol. 20, No. 12, 2000, p. 1077-1082.

Research output: Contribution to journalArticle

Ohe, H. ; Takashiba, S. ; Naruishi, K. ; Chou, H. H. ; Yamada, H. ; Nishimura, Fusanori ; Arai, H. ; Murayama, Y. / Tumor necrosis factor-α (TNF-α)-induced and interleukin-1β (IL-1β)-induced shedding of TNF receptors from gingival fibroblasts. In: Journal of Interferon and Cytokine Research. 2000 ; Vol. 20, No. 12. pp. 1077-1082.
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