Tumor regression by combined immunotherapy and hyperthermia using magnetic nanoparticles in an experimental subcutaneous murine melanoma

Akira Ito, Kouji Tanaka, Kazuyoshi Kondo, Masashige Shinkai, Hiroyuki Honda, Kazuhiko Matsumoto, Toshiaki Saida, Takeshi Kobayashi

Research output: Contribution to journalArticle

148 Citations (Scopus)

Abstract

Immunotherapy (IT) has become an accepted therapeutic modality. We previously reported that intracellular hyperthermia (IH) using magnetic nanoparticles induces antitumor immunity. We undertook these studies in order to study the combined effects of IT and IH on melanoma. Magnetite cationic liposomes (MCLs) have a positive surface charge and generate heat in an alternating magnetic field (AMF) due to hysteresis loss. MCLs were injected into a B16 melanoma nodule in C57BL/6 mice, which were subjected to AMF for 30 min. The temperature at the tumor reached 43°C and was maintained by controlling the magnetic field intensity. At 24 h after IH, interleukin-2 (IL-2) or granulocyte macrophage-colony stimulating factor (GM-CSF) was injected directly into the melanoma. Mice were divided into six groups: group I (control), group II (IH), group III (IL-2), group IV (GM-CSF), group V (IH+IL-2), and group VI (IH+GM-CSF). Complete regression of tumors was observed in mice of groups V and VI (75% (6/8) and 40% (4/10) of the mice, respectively), while no tumor regression was observed in mice of the other groups. This study supports the combined use of IT and IH using MCLs in patients with advanced malignancies.

Original languageEnglish
Pages (from-to)308-313
Number of pages6
JournalCancer Science
Volume94
Issue number3
DOIs
Publication statusPublished - Mar 1 2003

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Immunotherapy
Nanoparticles
Melanoma
Fever
Ferrosoferric Oxide
Magnetic Fields
Granulocyte-Macrophage Colony-Stimulating Factor
Neoplasms
Liposomes
Interleukin-2
Experimental Melanomas
Inbred C57BL Mouse
Immunity
Hot Temperature
Control Groups
Temperature

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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Tumor regression by combined immunotherapy and hyperthermia using magnetic nanoparticles in an experimental subcutaneous murine melanoma. / Ito, Akira; Tanaka, Kouji; Kondo, Kazuyoshi; Shinkai, Masashige; Honda, Hiroyuki; Matsumoto, Kazuhiko; Saida, Toshiaki; Kobayashi, Takeshi.

In: Cancer Science, Vol. 94, No. 3, 01.03.2003, p. 308-313.

Research output: Contribution to journalArticle

Ito, Akira ; Tanaka, Kouji ; Kondo, Kazuyoshi ; Shinkai, Masashige ; Honda, Hiroyuki ; Matsumoto, Kazuhiko ; Saida, Toshiaki ; Kobayashi, Takeshi. / Tumor regression by combined immunotherapy and hyperthermia using magnetic nanoparticles in an experimental subcutaneous murine melanoma. In: Cancer Science. 2003 ; Vol. 94, No. 3. pp. 308-313.
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