TY - JOUR
T1 - Tunable separations based on a molecular size effect for biomolecules by poly(ethylene glycol) gel-based capillary electrophoresis
AU - Kubo, Takuya
AU - Nishimura, Naoki
AU - Furuta, Hayato
AU - Kubota, Kei
AU - Naito, Toyohiro
AU - Otsuka, Koji
N1 - Funding Information:
This research was partly supported by the Grant-in Aid for Scientific Research ( 15K13756 ) from the Japan Society for the Promotion of Science . We thank Shin-Nakamura Chemical for a kind donation of PEGDMAs. Appendix A
PY - 2017/11/10
Y1 - 2017/11/10
N2 - We report novel capillary gel electrophoresis (CGE) with poly(ethylene glycol) (PEG)-based hydrogels for the effective separations of biomolecules containing sugars and DNAs based on a molecular size effect. The gel capillaries were prepared in a fused silica capillary modified with 3-(trimethoxysilyl)propylmethacrylate using a variety of the PEG-based hydrogels. After the fundamental evaluations in CGE regarding the separation based on the molecular size effect depending on the crosslinking density, the optimized capillary provided the efficient separation of glucose ladder (G1 to G20). In addition, another capillary showed the successful separation of DNA ladder in the range of 10–1100 base pair, which is superior to an authentic acrylamide-based gel capillary. For both glucose and DNA ladders, the separation ranges against the molecular size were simply controllable by alteration of the concentration and/or units of ethylene oxide in the PEG-based crosslinker. Finally, we demonstrated the separations of real samples, which included sugars carved out from monoclonal antibodies, mAbs, and then the efficient separations based on the molecular size effect were achieved.
AB - We report novel capillary gel electrophoresis (CGE) with poly(ethylene glycol) (PEG)-based hydrogels for the effective separations of biomolecules containing sugars and DNAs based on a molecular size effect. The gel capillaries were prepared in a fused silica capillary modified with 3-(trimethoxysilyl)propylmethacrylate using a variety of the PEG-based hydrogels. After the fundamental evaluations in CGE regarding the separation based on the molecular size effect depending on the crosslinking density, the optimized capillary provided the efficient separation of glucose ladder (G1 to G20). In addition, another capillary showed the successful separation of DNA ladder in the range of 10–1100 base pair, which is superior to an authentic acrylamide-based gel capillary. For both glucose and DNA ladders, the separation ranges against the molecular size were simply controllable by alteration of the concentration and/or units of ethylene oxide in the PEG-based crosslinker. Finally, we demonstrated the separations of real samples, which included sugars carved out from monoclonal antibodies, mAbs, and then the efficient separations based on the molecular size effect were achieved.
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U2 - 10.1016/j.chroma.2017.06.043
DO - 10.1016/j.chroma.2017.06.043
M3 - Article
C2 - 28645754
AN - SCOPUS:85020864121
VL - 1523
SP - 107
EP - 113
JO - Journal of Chromatography A
JF - Journal of Chromatography A
SN - 0021-9673
ER -