Tuning of cell proliferation on tough gels by critical charge effect

Mei Chen Yong, Jian Ping Gong, Masaru Tanaka, Kazunori Yasuda, Sadaaki Yamamoto, Masatsugu Shimomura, Yoshihito Osada

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Tough triple network (TN) hydrogels that facilitate cell spreading and proliferation and, at the same time, preserve high mechanical strength are synthesized by the introduction of a proper component of negatively charged moiety, poly(2-acrylamido-2-methyl-propane sulfonic acid sodium salt) (PNaAMPS), on which cells proliferate, with neutral moiety, poly(N,N-dimethylacrylamide) (DMAAm), on which cells do not proliferate, as the third network component, to PNaAMPS/PDMAAm double network (DN) gels. For synthesizing the tough TN gels to support cell viability, the effect of charge density on the behaviors of three kinds of cells, bovine fetal aorta endothelial cells (BFAECs), human umbilical endothelial cells (HUVECs), and rabbit synovial tissue-derived fibroblast cells (RSTFCs) were systematically investigated on poly(NaAMPS-co-DMAAm) gels with different charge density. The charge density of the gels was tuned by changing the molar fraction (F) of negatively charged monomer in the copolymer hydrogels. Critical F, which corresponds to a critical value of the zeta potential (ζ), is observed for cell spreading and proliferation. The critical F for BFAECs and HUVECs proliferate to confluent is F = 0.4 (ζ = -20 mV), whereas the critical F for RSTFCs shifts to F = 0.7 (ζ = -28.5 mV). The effect of gel charge density on cell behavior is correlated well with the total adsorbed proteins and fibronectin. By applying these results, cell proliferation is successfully realized on the tough TN hydrogels without surface modification with any cell adhesive proteins or peptides. The results will substantially promote the application of tough hydrogels as soft and wet tissues.

Original languageEnglish
Pages (from-to)74-83
Number of pages10
JournalJournal of Biomedical Materials Research - Part A
Volume88
Issue number1
DOIs
Publication statusPublished - Jan 1 2009
Externally publishedYes

Fingerprint

Cell proliferation
Hydrogels
Charge density
Gels
Tuning
Endothelial cells
Fibroblasts
Tissue
Cells
Proteins
Network components
Propane
Sulfonic Acids
Zeta potential
Fibronectins
Peptides
Strength of materials
Surface treatment
Adhesives
Copolymers

All Science Journal Classification (ASJC) codes

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys

Cite this

Tuning of cell proliferation on tough gels by critical charge effect. / Yong, Mei Chen; Gong, Jian Ping; Tanaka, Masaru; Yasuda, Kazunori; Yamamoto, Sadaaki; Shimomura, Masatsugu; Osada, Yoshihito.

In: Journal of Biomedical Materials Research - Part A, Vol. 88, No. 1, 01.01.2009, p. 74-83.

Research output: Contribution to journalArticle

Yong, Mei Chen ; Gong, Jian Ping ; Tanaka, Masaru ; Yasuda, Kazunori ; Yamamoto, Sadaaki ; Shimomura, Masatsugu ; Osada, Yoshihito. / Tuning of cell proliferation on tough gels by critical charge effect. In: Journal of Biomedical Materials Research - Part A. 2009 ; Vol. 88, No. 1. pp. 74-83.
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