Turbidimetric detection of target molecules using DNA aptamer-linked nanoparticles

Daisuke Miyamoto, Tohru Takarada, Zhonglan Tang, Mizuo Maeda

Research output: Contribution to conferencePaper

Abstract

We have recently found that the nanoparticles tethering single-stranded DNAs aggregated upon hybridization with complementary DNAs. In this study, we applied this phenomenon to the detection of ATP exploiting a DNA aptamer. Poly(N-isopropylacrylamide)-g-DNA was synthesized as previously reported and was dissolved in Tris-HCl buffer. Then, the buffer solution of the polymer was heated to 40°C to give the DNA-linked nanoparticles through the phase transition. Using this DNA-linked nanoparticles, we constructed two types of ATP detection system where the nanoparticles dispersed or aggregated only when the DNA aptamer recognized ATP. In one detection system, the nanoparticles aggregated in the absence of ATP and kept dispersing in the presence of ATP. In another system, the nanoparticles kept dispersing in the abesence of ATP and aggregated in the presence of ATP. The turbidity change in each detection system can be easily found with naked eye.

Original languageEnglish
Pages5122-5123
Number of pages2
Publication statusPublished - Dec 1 2005
Externally publishedYes
Event54th SPSJ Symposium on Macromolecules - Yamagata, Japan
Duration: Sep 20 2005Sep 22 2005

Other

Other54th SPSJ Symposium on Macromolecules
CountryJapan
CityYamagata
Period9/20/059/22/05

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All Science Journal Classification (ASJC) codes

  • Engineering(all)

Cite this

Miyamoto, D., Takarada, T., Tang, Z., & Maeda, M. (2005). Turbidimetric detection of target molecules using DNA aptamer-linked nanoparticles. 5122-5123. Paper presented at 54th SPSJ Symposium on Macromolecules, Yamagata, Japan.