Twinkle overexpression prevents cardiac rupture after myocardial infarction by alleviating impaired mitochondrial biogenesis

Takahiro Inoue, Masataka Ikeda, Tomomi Ide, Takeo Fujino, Yuka Matsuo, Shinobu Arai, Keita Saku, Kenji Sunagawa

Research output: Contribution to journalArticle

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Abstract

Cardiac rupture is a fatal complication after myocardial infarction (MI). However, the detailed mechanism underlying cardiac rupture after MI remains to be fully elucidated. In this study, we investigated the role of mitochondrial DNA (mtDNA) and mitochondria in the pathophysiology of cardiac rupture by analyzing Twinkle helicase overexpression mice (TW mice). Twinkle overexpression increased mtDNA copy number approximately twofold and ameliorated ischemic cardiomyopathy at day 28 after MI. Notably, Twinkle overexpression markedly prevented cardiac rupture and improved post-MI survival, accompanied by the suppression of MMP-2 and MMP-9 in the MI border area at day 5 after MI when cardiac rupture frequently occurs. Additionally, these cardioprotective effects of Twinkle overexpression were abolished in transgenic mice overexpressing mutant Twinkle with an in-frame duplication of amino acids 353–365, which resulted in no increases in mtDNA copy number. Furthermore, although apoptosis and oxidative stress were induced and mitochondria were damaged in the border area, these injuries were improved in TW mice. Further analysis revealed that mitochondrial biogenesis, including mtDNA copy number, transcription, and translation, was severely impaired in the border area at day 5. In contrast, Twinkle overexpression maintained mtDNA copy number and restored the impaired transcription and translation of mtDNA in the border area. These results demonstrated that Twinkle overexpression alleviated impaired mitochondrial biogenesis in the border area through maintained mtDNA copy number and thereby prevented cardiac rupture accompanied by the reduction of apoptosis and oxidative stress, and suppression of MMP activity.

Original languageEnglish
Pages (from-to)H509-H519
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume311
Issue number3
DOIs
Publication statusPublished - Sep 1 2016

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Heart Rupture
Organelle Biogenesis
Mitochondrial DNA
Myocardial Infarction
Matrix Metalloproteinases
Mitochondria
Oxidative Stress
Apoptosis
Cardiomyopathies
Transgenic Mice
Amino Acids
Wounds and Injuries

All Science Journal Classification (ASJC) codes

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Twinkle overexpression prevents cardiac rupture after myocardial infarction by alleviating impaired mitochondrial biogenesis. / Inoue, Takahiro; Ikeda, Masataka; Ide, Tomomi; Fujino, Takeo; Matsuo, Yuka; Arai, Shinobu; Saku, Keita; Sunagawa, Kenji.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 311, No. 3, 01.09.2016, p. H509-H519.

Research output: Contribution to journalArticle

Inoue, Takahiro ; Ikeda, Masataka ; Ide, Tomomi ; Fujino, Takeo ; Matsuo, Yuka ; Arai, Shinobu ; Saku, Keita ; Sunagawa, Kenji. / Twinkle overexpression prevents cardiac rupture after myocardial infarction by alleviating impaired mitochondrial biogenesis. In: American Journal of Physiology - Heart and Circulatory Physiology. 2016 ; Vol. 311, No. 3. pp. H509-H519.
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