Two-component systems involved in susceptibility to nisin A in Streptococcus pyogenes

Miki Kawada-Matsuo, Ichiro Tatsuno, Kaoru Arii, Takeshi Zendo, Yuichi Oogai, Kazuyuki Noguchi, Tadao Hasegawa, Kenji Sonomoto, Hitoshi Komatsuzawa

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Two-component systems (TCSs) are regulatory systems in bacteria that play important roles in sensing and adapting to the environment. In this study, we systematically evaluated the roles of TCSs in the susceptibility of the group A Streptococcus (GAS; Streptococcus pyogenes) SF370 strain to several types of lantibiotics. Using individual TCS deletion mutants, we found that the deletion of srtRK (spy_1081-spy_1082) in SF370 increased the susceptibility to nisin A, which is produced by Lactococcus lactis ATCC 11454, but susceptibility to other types of lantibiotics (nukacin ISK-1, produced by Staphylococcus warneri, and staphylococcin C55, produced by Staphylococcus aureus) was not altered in the TCS mutants tested. The expression of srtFEG (spy_1085 to spy_1087), which is located downstream of srtRK and is homologous to ABC transporters, was increased in response to nisin A. However, srtEFG expression was not induced by nisin A in the srtRK mutant. The inactivation of srtFEG increased the susceptibility to nisin A. These results suggest that SrtRK controls SrtFEG expression to alter the susceptibility to nisin A. Further experiments showed that SrtRK is required for coexistence with L. lactis ATCC 11454, which produces nisin A. Our results elucidate the important roles of S. pyogenes TCSs in the interactions between different bacterial species, including bacteriocinproducing bacteria.

Original languageEnglish
Pages (from-to)5930-5939
Number of pages10
JournalApplied and environmental microbiology
Volume82
Issue number19
DOIs
Publication statusPublished - 2016

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

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