Abstract
d-Alanine (d-Ala) is one of the naturally occurring d-amino acids in mammals, and its amount is known to have characteristic circadian changes. It is a candidate for a novel physiologically active substance and/or a biomarker, and the regulation mechanisms of the intrinsic amounts of d-Ala are expected to be clarified. In the present study, the effects of the possible factors controlling the d-Ala amounts, e.g., diet, d-amino acid oxidase (DAO) and intestinal bacteria, on the day-night changes in the intrinsic d-Ala amounts have been investigated using a highly sensitive and selective two-dimensional high-performance liquid chromatographic system combining a reversed-phase column and an enantioselective column. The circadian rhythm was not changed under fasting conditions. In the mice lacking d-amino acid oxidase activity (ddY/DAO- mice), clear day-night changes were still observed, suggesting that the factors controlling the d-Ala rhythm were not their food and DAO activity. On the other hand, in the germ-free mice, quite low amounts of d-Ala were detected compared with those in the control mice, indicating that the main origin of d-Ala in the mice is intestinal bacteria. Because the d-Ala amounts in the digesta containing intestinal bacteria did not show the day-night changes, the controlling factor of the circadian changes of the d-Ala amount was suggested to be the intestinal absorption.
| Original language | English |
|---|---|
| Pages (from-to) | 8083-8091 |
| Number of pages | 9 |
| Journal | Analytical and Bioanalytical Chemistry |
| Volume | 405 |
| Issue number | 25 |
| DOIs | |
| Publication status | Published - Oct 1 2013 |
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All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Biochemistry
Cite this
Two-dimensional high-performance liquid chromatographic determination of day-night variation of d-alanine in mammals and factors controlling the circadian changes Amino Acid Analysis. / Karakawa, Sachise; Miyoshi, Yurika; Konno, Ryuichi; Koyanagi, Satoru; Mita, Masashi; Ohdo, Shigehiro; Hamase, Kenji.
In: Analytical and Bioanalytical Chemistry, Vol. 405, No. 25, 01.10.2013, p. 8083-8091.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Two-dimensional high-performance liquid chromatographic determination of day-night variation of d-alanine in mammals and factors controlling the circadian changes Amino Acid Analysis
AU - Karakawa, Sachise
AU - Miyoshi, Yurika
AU - Konno, Ryuichi
AU - Koyanagi, Satoru
AU - Mita, Masashi
AU - Ohdo, Shigehiro
AU - Hamase, Kenji
PY - 2013/10/1
Y1 - 2013/10/1
N2 - d-Alanine (d-Ala) is one of the naturally occurring d-amino acids in mammals, and its amount is known to have characteristic circadian changes. It is a candidate for a novel physiologically active substance and/or a biomarker, and the regulation mechanisms of the intrinsic amounts of d-Ala are expected to be clarified. In the present study, the effects of the possible factors controlling the d-Ala amounts, e.g., diet, d-amino acid oxidase (DAO) and intestinal bacteria, on the day-night changes in the intrinsic d-Ala amounts have been investigated using a highly sensitive and selective two-dimensional high-performance liquid chromatographic system combining a reversed-phase column and an enantioselective column. The circadian rhythm was not changed under fasting conditions. In the mice lacking d-amino acid oxidase activity (ddY/DAO- mice), clear day-night changes were still observed, suggesting that the factors controlling the d-Ala rhythm were not their food and DAO activity. On the other hand, in the germ-free mice, quite low amounts of d-Ala were detected compared with those in the control mice, indicating that the main origin of d-Ala in the mice is intestinal bacteria. Because the d-Ala amounts in the digesta containing intestinal bacteria did not show the day-night changes, the controlling factor of the circadian changes of the d-Ala amount was suggested to be the intestinal absorption.
AB - d-Alanine (d-Ala) is one of the naturally occurring d-amino acids in mammals, and its amount is known to have characteristic circadian changes. It is a candidate for a novel physiologically active substance and/or a biomarker, and the regulation mechanisms of the intrinsic amounts of d-Ala are expected to be clarified. In the present study, the effects of the possible factors controlling the d-Ala amounts, e.g., diet, d-amino acid oxidase (DAO) and intestinal bacteria, on the day-night changes in the intrinsic d-Ala amounts have been investigated using a highly sensitive and selective two-dimensional high-performance liquid chromatographic system combining a reversed-phase column and an enantioselective column. The circadian rhythm was not changed under fasting conditions. In the mice lacking d-amino acid oxidase activity (ddY/DAO- mice), clear day-night changes were still observed, suggesting that the factors controlling the d-Ala rhythm were not their food and DAO activity. On the other hand, in the germ-free mice, quite low amounts of d-Ala were detected compared with those in the control mice, indicating that the main origin of d-Ala in the mice is intestinal bacteria. Because the d-Ala amounts in the digesta containing intestinal bacteria did not show the day-night changes, the controlling factor of the circadian changes of the d-Ala amount was suggested to be the intestinal absorption.
UR - http://www.scopus.com/inward/record.url?scp=84885293204&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84885293204&partnerID=8YFLogxK
U2 - 10.1007/s00216-013-7071-2
DO - 10.1007/s00216-013-7071-2
M3 - Article
C2 - 23732868
AN - SCOPUS:84885293204
VL - 405
SP - 8083
EP - 8091
JO - Fresenius Zeitschrift fur Analytische Chemie
JF - Fresenius Zeitschrift fur Analytische Chemie
SN - 0016-1152
IS - 25
ER -