Two diverged complement factor B/C2-like cDNA sequences from a teleost, the common carp (Cyprinus carpio)

Miki Nakao, Yuko Fushitani, Kazuhiro Fujiki, Masaru Nonaka, Tomoki Yano

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Mammalian complement components factor B and C2 act as proteolytic subunits of the C3 convertases in the alternative and the classical activation pathways, respectively, and are believed to have diverged from a common ancestor by gene duplication. However, it is unclear when the B/C2 duplication occurred. Here, we describe two diverged B/C2-like cDNA clones (B/C2-A and B/C2-B) isolated from a bony fish, the common carp (Cyprinus carpio). B/C2-A shares the same domain structure as the factor B and C2 complement components of vertebrates reported so far and shows a close similarity to zebrafish B and medaka fish B/C2. These teleost sequences show almost the same degree of similarity to C2 and B of higher vertebrates. In contrast, B/C2-B has a novel structural feature in thai it contains four short consensus repeat modules and does not have a close relative upon phylogenetic analysis. Northern blotting revealed the presence of two transcripts with different sizes for both the B/C2-A and B/C2-B in the hepatopancreas of the carp. Southern blotting suggested the presence of multiple genes for B/C2-A and a single gene for B/C2-B. Although structural features of B/C2-B are slightly more C2-like than B-like, B/C2-B has a crucial amino acid substitution in the serine protease domain, which makes it unlikely that B/C2-B functions as a C3 convertase. A possible phylogenetic relationship between the two carp sequences and mammalian C2 and B is discussed.

Original languageEnglish
Pages (from-to)4811-4818
Number of pages8
JournalJournal of Immunology
Volume161
Issue number9
Publication statusPublished - Nov 1 1998

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Complement Factor B
Carps
Complementary DNA
Complement C3-C5 Convertases
Vertebrates
Fishes
Oryzias
Hepatopancreas
Gene Duplication
Serine Proteases
Zebrafish
Amino Acid Substitution
Southern Blotting
Northern Blotting
Genes
Clone Cells

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

Two diverged complement factor B/C2-like cDNA sequences from a teleost, the common carp (Cyprinus carpio). / Nakao, Miki; Fushitani, Yuko; Fujiki, Kazuhiro; Nonaka, Masaru; Yano, Tomoki.

In: Journal of Immunology, Vol. 161, No. 9, 01.11.1998, p. 4811-4818.

Research output: Contribution to journalArticle

Nakao, M, Fushitani, Y, Fujiki, K, Nonaka, M & Yano, T 1998, 'Two diverged complement factor B/C2-like cDNA sequences from a teleost, the common carp (Cyprinus carpio)', Journal of Immunology, vol. 161, no. 9, pp. 4811-4818.
Nakao, Miki ; Fushitani, Yuko ; Fujiki, Kazuhiro ; Nonaka, Masaru ; Yano, Tomoki. / Two diverged complement factor B/C2-like cDNA sequences from a teleost, the common carp (Cyprinus carpio). In: Journal of Immunology. 1998 ; Vol. 161, No. 9. pp. 4811-4818.
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