Two homologous agr-like quorum-sensing systems cooperatively control adherence, cell morphology, and cell viability properties in Lactobacillus plantarum WCFS1

Toshio Fujii, Colin Ingham, Jiro Nakayama, Marke Beerthuyzen, Ryoko Kunuki, Douwe Molenaar, Mark Sturme, Elaine Vaughan, Michiel Kleerebezem, Willem De Vos

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Abstract

A two-component regulatory system of Lactobacillus plantarum, encoded by genes designated lamK and lamR (hpk10 and rrp10), was studied. The lamK and lamR genes encode proteins which are highly homologous to the quorum-sensing histidine kinase LamC and the response regulator LamA, respectively. Transcription analysis of the lamKR operon and the lamBDCA operon and liquid chromatography-mass spectrometry analysis of production of the LamD558 autoinducing peptide were performed for ΔlamA, ΔlamR, ΔlamA ΔlamR deletion mutants and a wild-type strain. The results suggested that lamA and lamR are cooperating genes. In addition, typical phenotypes of the ΔlamA mutant, such as reduced adherence to glass surfaces and filamentous cell morphology, were enhanced in the ΔlamA ΔlamR mutant. Microarray analysis suggested that the same cell wall polysaccharide synthesis genes, stress response-related genes, and cell wall protein-encoding genes were affected in the ΔlamA and ΔlamA ΔlamR mutants. However, the regulation ratio was more significant for the ΔlamA ΔlamR mutant, indicating the cooperative effect of LamA and LamR.

Original languageEnglish
Pages (from-to)7655-7665
Number of pages11
JournalJournal of bacteriology
Volume190
Issue number23
DOIs
Publication statusPublished - Dec 1 2008

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Lactobacillus plantarum
Quorum Sensing
Cell Survival
Operon
Cell Wall
Genes
Microarray Analysis
Liquid Chromatography
Glass
Polysaccharides
Mass Spectrometry
Proteins
Phenotype
Peptides

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

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Two homologous agr-like quorum-sensing systems cooperatively control adherence, cell morphology, and cell viability properties in Lactobacillus plantarum WCFS1. / Fujii, Toshio; Ingham, Colin; Nakayama, Jiro; Beerthuyzen, Marke; Kunuki, Ryoko; Molenaar, Douwe; Sturme, Mark; Vaughan, Elaine; Kleerebezem, Michiel; De Vos, Willem.

In: Journal of bacteriology, Vol. 190, No. 23, 01.12.2008, p. 7655-7665.

Research output: Contribution to journalArticle

Fujii, T, Ingham, C, Nakayama, J, Beerthuyzen, M, Kunuki, R, Molenaar, D, Sturme, M, Vaughan, E, Kleerebezem, M & De Vos, W 2008, 'Two homologous agr-like quorum-sensing systems cooperatively control adherence, cell morphology, and cell viability properties in Lactobacillus plantarum WCFS1', Journal of bacteriology, vol. 190, no. 23, pp. 7655-7665. https://doi.org/10.1128/JB.01489-07
Fujii, Toshio ; Ingham, Colin ; Nakayama, Jiro ; Beerthuyzen, Marke ; Kunuki, Ryoko ; Molenaar, Douwe ; Sturme, Mark ; Vaughan, Elaine ; Kleerebezem, Michiel ; De Vos, Willem. / Two homologous agr-like quorum-sensing systems cooperatively control adherence, cell morphology, and cell viability properties in Lactobacillus plantarum WCFS1. In: Journal of bacteriology. 2008 ; Vol. 190, No. 23. pp. 7655-7665.
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