TY - JOUR
T1 - Two leptin genes and a leptin receptor gene of female chub mackerel (Scomber japonicus)
T2 - Molecular cloning, tissue distribution and expression in different obesity indices and pubertal stages
AU - Ohga, Hirofumi
AU - Matsumori, Kojiro
AU - Kodama, Ryoko
AU - Kitano, Hajime
AU - Nagano, Naoki
AU - Yamaguchi, Akihiko
AU - Matsuyama, Michiya
N1 - Funding Information:
We are also grateful to Takakushi-Shōeimaru Co., Ltd. and Kyōyō-Katsugyo Co., Ltd. for fish care and provide the samples. We thank to Hayato Adachi and Sanny David. P. Lumayno for their assistance during fish sampling. This study was performed as part of the Technological Development for Selection and Secure Stock of Bloodstock for Culture of Bluefin Tuna, which is supported by the Fisheries Agency of Japan .
Publisher Copyright:
© 2015 Elsevier Inc.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2015/10/1
Y1 - 2015/10/1
N2 - Leptin is a hormone produced by fat cells that regulates the amount of fat stored in the body and conveys nutritional status to the reproductive axis in mammals. In the present study we identified two subtypes of leptin genes (lepa and lepb) and a leptin receptor gene (lepr) from chub mackerel (Scomber japonicus) and there gene expression under different feeding conditions (control and high-feed) and pubertal development stages was analyzed using quantitative real-time PCR. The protein lengths of LepA, LepB and LepR were 161 amino acids (aa), 163 aa and 1149 aa, respectively and both leptin subtypes shared only 15% similarity in aa sequences. In pubertal females, lepa was expressed in the brain, pituitary gland, liver, adipose tissue and ovary; however, in adult (gonadal maturation after the second in the life) females, lepa was expressed only in the liver. lepb was expressed primarily in the brain of all fish tested and was expressed strongly in the adipose tissue of adults. lepr was characterized by expression in the pituitary. The high-feed group showed a high conditioning factor level; unexpectedly, hepatic lepa and brain lepr were significantly more weakly expressed compared with the control-feed group. Furthermore, the expression levels of lepa, lepb and lepr genes showed no significant differences between pre-pubertal and post-pubertal fish. On the other hand, pituitary fshβ and lhβ showed no significant differences between different feeding groups of pre-pubertal fish. In contrast, fshβ and lhβ expressed abundantly in the post-pubertal fish of control feed group. Based on these results, whether leptin plays an important role in the nutritional status and pubertal onset of chub mackerel remains unknown.
AB - Leptin is a hormone produced by fat cells that regulates the amount of fat stored in the body and conveys nutritional status to the reproductive axis in mammals. In the present study we identified two subtypes of leptin genes (lepa and lepb) and a leptin receptor gene (lepr) from chub mackerel (Scomber japonicus) and there gene expression under different feeding conditions (control and high-feed) and pubertal development stages was analyzed using quantitative real-time PCR. The protein lengths of LepA, LepB and LepR were 161 amino acids (aa), 163 aa and 1149 aa, respectively and both leptin subtypes shared only 15% similarity in aa sequences. In pubertal females, lepa was expressed in the brain, pituitary gland, liver, adipose tissue and ovary; however, in adult (gonadal maturation after the second in the life) females, lepa was expressed only in the liver. lepb was expressed primarily in the brain of all fish tested and was expressed strongly in the adipose tissue of adults. lepr was characterized by expression in the pituitary. The high-feed group showed a high conditioning factor level; unexpectedly, hepatic lepa and brain lepr were significantly more weakly expressed compared with the control-feed group. Furthermore, the expression levels of lepa, lepb and lepr genes showed no significant differences between pre-pubertal and post-pubertal fish. On the other hand, pituitary fshβ and lhβ showed no significant differences between different feeding groups of pre-pubertal fish. In contrast, fshβ and lhβ expressed abundantly in the post-pubertal fish of control feed group. Based on these results, whether leptin plays an important role in the nutritional status and pubertal onset of chub mackerel remains unknown.
UR - http://www.scopus.com/inward/record.url?scp=84960314837&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84960314837&partnerID=8YFLogxK
U2 - 10.1016/j.ygcen.2015.06.002
DO - 10.1016/j.ygcen.2015.06.002
M3 - Article
C2 - 26065595
AN - SCOPUS:84960314837
SN - 0016-6480
VL - 222
SP - 88
EP - 98
JO - General and Comparative Endocrinology
JF - General and Comparative Endocrinology
ER -
