Two-peptide bacteriocin from Lactobacillus plantarum PMU 33 strain isolated from som-fak, a Thai low salt fermented fsh product

W. Noonpakdee, P. Jumriangrit, K. Wittayakom, Takeshi Zendo, Jiro Nakayama, Kenji Sonomoto, S. Panyim

Research output: Contribution to journalArticle

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Abstract

A total of 12,520 lactic acid bacteria (LAB) isolated from fermented fsh products "som-fak" were screened for bacteriocin. One Lactobacillus plantarum PMU33 strain produced bacteriocin that inhibited a large number of Gram-positive bacteria including food borne pathogens, Listeria monocytogenes, Bacillus cereus and Staphylococcus aureus. Biochemical studies revealed that the bacteriocin was heat stable even at autoclaving temperature (121°C for 15 min) and was active over a wide pH range (2-10). The bacteriocin purifed and characterized from the culture supernatant consists of two peptides with the molecular masses of 3222 and 3099 by mass spectrometry analysis. The molecular mass of this two-peptide bacteriocin were nearly identical to that of two-peptide plantaricin W (Plw) which consists of two peptides Plwa and Plwβ. The genes encoding these two peptides amplifed by PCR with Plw gene specifc primer showed identical sequences to Plwa and Plwβ. The bacteriocins and their producing strains isolated from som-fak may fnd application as bio-preservatives in fermented food products.

Original languageEnglish
Pages (from-to)19-25
Number of pages7
JournalAsia-Pacific Journal of Molecular Biology and Biotechnology
Volume17
Issue number1
Publication statusPublished - 2009

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Lactobacillus plantarum
Bacteriocins
Salts
Peptides
Food
Bacillus cereus
Listeria monocytogenes
Gram-Positive Bacteria
Genes
Staphylococcus aureus
Lactic Acid
Mass Spectrometry
Hot Temperature
Bacteria
Polymerase Chain Reaction
Temperature

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Molecular Biology

Cite this

Two-peptide bacteriocin from Lactobacillus plantarum PMU 33 strain isolated from som-fak, a Thai low salt fermented fsh product. / Noonpakdee, W.; Jumriangrit, P.; Wittayakom, K.; Zendo, Takeshi; Nakayama, Jiro; Sonomoto, Kenji; Panyim, S.

In: Asia-Pacific Journal of Molecular Biology and Biotechnology, Vol. 17, No. 1, 2009, p. 19-25.

Research output: Contribution to journalArticle

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AU - Jumriangrit, P.

AU - Wittayakom, K.

AU - Zendo, Takeshi

AU - Nakayama, Jiro

AU - Sonomoto, Kenji

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