Two polymorphisms within interleukin-3 (hlL3) gene detected by mismatch PCR/RFLP

H. Nakashima, T. Otsuka, Y. Ohba, Mitsuteru Akahoshi, S. Nagano, E. Ogami, Yojiro Arinobu, K. Miyake, Y. Inoue, Hiroaki Niiro, Y. Kaji, Y. Niho

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Two alleles of IL-3 have been reported to GenBank (GenBank M14743, M20137). The sequence difference between these two alleles is at the first nucleotide of the 27th codon (the 131st nucleotide from the initiation site): thymine and cytosine, and leading the amino acid difference: proline and serine (Pro27Ser). The other allelism, thymine and cytosine, was also observed at position -16 of the IL-3 upstream promotor region (GenBank L10616, M60870). We clarified that these substitutions were frequent polymorphisms in the Japanese population by using the mismatch-PCR (polymerase chain reaction)/RFLP (restriction fragment length polymorphism) method.

Original languageEnglish
Pages (from-to)156-158
Number of pages3
JournalGenes and Immunity
Volume1
Issue number2
DOIs
Publication statusPublished - Jan 1 1999

Fingerprint

Interleukin-3
Nucleic Acid Databases
Restriction Fragment Length Polymorphisms
Thymine
Cytosine
Polymerase Chain Reaction
Nucleotides
Alleles
Genes
Genetic Promoter Regions
Proline
Codon
Serine
Amino Acids
Population

All Science Journal Classification (ASJC) codes

  • Immunology
  • Genetics
  • Genetics(clinical)

Cite this

Two polymorphisms within interleukin-3 (hlL3) gene detected by mismatch PCR/RFLP. / Nakashima, H.; Otsuka, T.; Ohba, Y.; Akahoshi, Mitsuteru; Nagano, S.; Ogami, E.; Arinobu, Yojiro; Miyake, K.; Inoue, Y.; Niiro, Hiroaki; Kaji, Y.; Niho, Y.

In: Genes and Immunity, Vol. 1, No. 2, 01.01.1999, p. 156-158.

Research output: Contribution to journalArticle

Nakashima, H, Otsuka, T, Ohba, Y, Akahoshi, M, Nagano, S, Ogami, E, Arinobu, Y, Miyake, K, Inoue, Y, Niiro, H, Kaji, Y & Niho, Y 1999, 'Two polymorphisms within interleukin-3 (hlL3) gene detected by mismatch PCR/RFLP', Genes and Immunity, vol. 1, no. 2, pp. 156-158. https://doi.org/10.1038/sj.gene.6363636
Nakashima, H. ; Otsuka, T. ; Ohba, Y. ; Akahoshi, Mitsuteru ; Nagano, S. ; Ogami, E. ; Arinobu, Yojiro ; Miyake, K. ; Inoue, Y. ; Niiro, Hiroaki ; Kaji, Y. ; Niho, Y. / Two polymorphisms within interleukin-3 (hlL3) gene detected by mismatch PCR/RFLP. In: Genes and Immunity. 1999 ; Vol. 1, No. 2. pp. 156-158.
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AU - Nagano, S.

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AU - Arinobu, Yojiro

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