Two polymorphisms within interleukin-3 (hlL3) gene detected by mismatch PCR/RFLP

H. Nakashima; T. Otsuka; Y. Ohba; M. Akahoshi; S. Nagano; E. Ogami; Y. Arinobu; K. Miyake; Y. Inoue; H. Niiro; Y. Kaji; Y. Niho

doi:10.1038/sj.gene.6363636

Two polymorphisms within interleukin-3 (hlL3) gene detected by mismatch PCR/RFLP

H. Nakashima, T. Otsuka, Y. Ohba, M. Akahoshi, S. Nagano, E. Ogami, Y. Arinobu, K. Miyake, Y. Inoue, H. Niiro, Y. Kaji, Y. Niho

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2 Citations (Scopus)

Abstract

Two alleles of IL-3 have been reported to GenBank (GenBank M14743, M20137). The sequence difference between these two alleles is at the first nucleotide of the 27th codon (the 131st nucleotide from the initiation site): thymine and cytosine, and leading the amino acid difference: proline and serine (Pro27Ser). The other allelism, thymine and cytosine, was also observed at position -16 of the IL-3 upstream promotor region (GenBank L10616, M60870). We clarified that these substitutions were frequent polymorphisms in the Japanese population by using the mismatch-PCR (polymerase chain reaction)/RFLP (restriction fragment length polymorphism) method.

Original language	English
Pages (from-to)	156-158
Number of pages	3
Journal	Genes and Immunity
Volume	1
Issue number	2
DOIs	https://doi.org/10.1038/sj.gene.6363636
Publication status	Published - Nov 1999

All Science Journal Classification (ASJC) codes

Immunology
Genetics
Genetics(clinical)

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title = "Two polymorphisms within interleukin-3 (hlL3) gene detected by mismatch PCR/RFLP",

abstract = "Two alleles of IL-3 have been reported to GenBank (GenBank M14743, M20137). The sequence difference between these two alleles is at the first nucleotide of the 27th codon (the 131st nucleotide from the initiation site): thymine and cytosine, and leading the amino acid difference: proline and serine (Pro27Ser). The other allelism, thymine and cytosine, was also observed at position -16 of the IL-3 upstream promotor region (GenBank L10616, M60870). We clarified that these substitutions were frequent polymorphisms in the Japanese population by using the mismatch-PCR (polymerase chain reaction)/RFLP (restriction fragment length polymorphism) method.",

author = "H. Nakashima and T. Otsuka and Y. Ohba and M. Akahoshi and S. Nagano and E. Ogami and Y. Arinobu and K. Miyake and Y. Inoue and H. Niiro and Y. Kaji and Y. Niho",

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doi = "10.1038/sj.gene.6363636",

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TY - JOUR

T1 - Two polymorphisms within interleukin-3 (hlL3) gene detected by mismatch PCR/RFLP

AU - Nakashima, H.

AU - Otsuka, T.

AU - Ohba, Y.

AU - Akahoshi, M.

AU - Nagano, S.

AU - Ogami, E.

AU - Arinobu, Y.

AU - Miyake, K.

AU - Inoue, Y.

AU - Niiro, H.

AU - Kaji, Y.

AU - Niho, Y.

PY - 1999/11

Y1 - 1999/11

N2 - Two alleles of IL-3 have been reported to GenBank (GenBank M14743, M20137). The sequence difference between these two alleles is at the first nucleotide of the 27th codon (the 131st nucleotide from the initiation site): thymine and cytosine, and leading the amino acid difference: proline and serine (Pro27Ser). The other allelism, thymine and cytosine, was also observed at position -16 of the IL-3 upstream promotor region (GenBank L10616, M60870). We clarified that these substitutions were frequent polymorphisms in the Japanese population by using the mismatch-PCR (polymerase chain reaction)/RFLP (restriction fragment length polymorphism) method.

AB - Two alleles of IL-3 have been reported to GenBank (GenBank M14743, M20137). The sequence difference between these two alleles is at the first nucleotide of the 27th codon (the 131st nucleotide from the initiation site): thymine and cytosine, and leading the amino acid difference: proline and serine (Pro27Ser). The other allelism, thymine and cytosine, was also observed at position -16 of the IL-3 upstream promotor region (GenBank L10616, M60870). We clarified that these substitutions were frequent polymorphisms in the Japanese population by using the mismatch-PCR (polymerase chain reaction)/RFLP (restriction fragment length polymorphism) method.

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JO - Genes and Immunity

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Two polymorphisms within interleukin-3 (hlL3) gene detected by mismatch PCR/RFLP

Abstract

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