Two relatively distinct patterns of ameloblastoma: An anti-apoptotic proliferating site in the outer layer (periphery) and a pro-apoptotic differentiating site in the inner layer (centre)

F. Sandra, N. Nakamura, Takeshi Mitsuyasu, Y. Shiratsuchi, M. Ohishi

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Abstract

Aims: This study was performed to determine the apoptotic behaviour of ameloblastomas by analysing the role of bcl-2 family proteins in ameloblastomas and the location of terminally apoptotic cells in the amelo-blastoma epithelial tissues. Methods and results: For immunohistochemistry, tissue sections of 32 patients were treated with an antigen-retrieval method. Primary antibodies against the apoptosis-related proteins, bcl-2, bcl-X, bax, and bak were applied. Besides immunohistochemistry, Western blotting and TUNEL were also performed. Most of the outer layer cells were predominantly stained by the bcl-2 antibody, while most of the inner layer cells were stained by antibodies against the apoptosis-modulating proteins, bax and bak. Among the bcl-2 family, bcl-2 was the most ubiquitously expressed protein in ameloblastomas, while bcl-X was expressed in the greatest concentrations. The major bcl-X protein was bcl-XL. Some of the inner layer cells entered the terminal apoptotic stage, which were revealed by TUNEL. The acanthomatous areas over-expressed the apoptosis-modulating proteins, especially bak. Conclusions: Ameloblastoma has much more apoptosis-inhibiting protein than the apoptosis-modulating protein. Ameloblastoma has two relatively distinct patterns, an anti-apoptotic proliferating site in the outer layer (periphery) and a pro-apoptotic differentiating site in the inner layer (centre). The acanthomatous area, which was stained strongly by bak antibody and contained numerous terminally apoptotic cells, was considered as the differentiated area.

Original languageEnglish
Pages (from-to)93-98
Number of pages6
JournalHistopathology
Volume39
Issue number1
DOIs
Publication statusPublished - Sep 26 2001

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Ameloblastoma
bcl-2 Homologous Antagonist-Killer Protein
Apoptosis
Antibodies
In Situ Nick-End Labeling
Proteins
bcl-X Protein
Immunohistochemistry
bcl-2-Associated X Protein
Apoptosis Regulatory Proteins
Epithelium
Western Blotting
Antigens

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Histology

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Two relatively distinct patterns of ameloblastoma : An anti-apoptotic proliferating site in the outer layer (periphery) and a pro-apoptotic differentiating site in the inner layer (centre). / Sandra, F.; Nakamura, N.; Mitsuyasu, Takeshi; Shiratsuchi, Y.; Ohishi, M.

In: Histopathology, Vol. 39, No. 1, 26.09.2001, p. 93-98.

Research output: Contribution to journalArticle

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abstract = "Aims: This study was performed to determine the apoptotic behaviour of ameloblastomas by analysing the role of bcl-2 family proteins in ameloblastomas and the location of terminally apoptotic cells in the amelo-blastoma epithelial tissues. Methods and results: For immunohistochemistry, tissue sections of 32 patients were treated with an antigen-retrieval method. Primary antibodies against the apoptosis-related proteins, bcl-2, bcl-X, bax, and bak were applied. Besides immunohistochemistry, Western blotting and TUNEL were also performed. Most of the outer layer cells were predominantly stained by the bcl-2 antibody, while most of the inner layer cells were stained by antibodies against the apoptosis-modulating proteins, bax and bak. Among the bcl-2 family, bcl-2 was the most ubiquitously expressed protein in ameloblastomas, while bcl-X was expressed in the greatest concentrations. The major bcl-X protein was bcl-XL. Some of the inner layer cells entered the terminal apoptotic stage, which were revealed by TUNEL. The acanthomatous areas over-expressed the apoptosis-modulating proteins, especially bak. Conclusions: Ameloblastoma has much more apoptosis-inhibiting protein than the apoptosis-modulating protein. Ameloblastoma has two relatively distinct patterns, an anti-apoptotic proliferating site in the outer layer (periphery) and a pro-apoptotic differentiating site in the inner layer (centre). The acanthomatous area, which was stained strongly by bak antibody and contained numerous terminally apoptotic cells, was considered as the differentiated area.",
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