Two sox9 genes on duplicated zebrafish chromosomes: Expression of similar transcription activators in distinct sites

Evelyn F.L. Chiang, Chin I. Pai, Mary Wyatt, Yi Lin Yan, John Postlethwait, Bon Chu Chung

Research output: Contribution to journalArticle

218 Citations (Scopus)

Abstract

Sox9 is a transcription factor required for cartilage formation and testis determination in mammals. We have cloned from zebrafish two sox9 genes, termed sox9a and sox9b. Gene phylogenies showed that both genes are orthologous to tetrapod SOX9 genes. Genetic mapping showed that these two loci reside on chromosome segments that were apparently duplicated in a large-scale genomic duplication event in ray fin fish phylogeny. Both Sox9a and Sox9b proteins bind to the HMG consensus DNA sequences in vitro. We tested different domains for transactivation potential and identified a potential activation domain located in the middle of both Sox9a and Sox9b. During embryogenesis, sox9a and sox9b expression patterns are distinct but overlap in some regions of the brain, head skeleton, and fins. Expression of sox9a/b correlates well with that of col2a1 in chondrogenic elements. In the adults, sox9a is expressed in many tissues including brain, muscle, fin, and testis, whereas sox9b expression is restricted to previtellogenic oocytes of the ovary. This expression pattern predicts that sox9a and sox9b may have unique functions in some specific tissues during development. The role of gene duplication for the evolution of developmental gene function is discussed.

Original languageEnglish
Pages (from-to)149-163
Number of pages15
JournalDevelopmental Biology
Volume231
Issue number1
DOIs
Publication statusPublished - Mar 1 2001

Fingerprint

Zebrafish
Chromosomes
Phylogeny
Genes
Testis
Skates (Fish)
Developmental Genes
Gene Duplication
Consensus Sequence
Brain
Skeleton
Transcriptional Activation
Cartilage
Oocytes
Embryonic Development
Mammals
Ovary
Transcription Factors
Head
Muscles

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Cite this

Two sox9 genes on duplicated zebrafish chromosomes : Expression of similar transcription activators in distinct sites. / Chiang, Evelyn F.L.; Pai, Chin I.; Wyatt, Mary; Yan, Yi Lin; Postlethwait, John; Chung, Bon Chu.

In: Developmental Biology, Vol. 231, No. 1, 01.03.2001, p. 149-163.

Research output: Contribution to journalArticle

Chiang, Evelyn F.L. ; Pai, Chin I. ; Wyatt, Mary ; Yan, Yi Lin ; Postlethwait, John ; Chung, Bon Chu. / Two sox9 genes on duplicated zebrafish chromosomes : Expression of similar transcription activators in distinct sites. In: Developmental Biology. 2001 ; Vol. 231, No. 1. pp. 149-163.
@article{14f78f91dedb4e4d9ce904e5f01526d8,
title = "Two sox9 genes on duplicated zebrafish chromosomes: Expression of similar transcription activators in distinct sites",
abstract = "Sox9 is a transcription factor required for cartilage formation and testis determination in mammals. We have cloned from zebrafish two sox9 genes, termed sox9a and sox9b. Gene phylogenies showed that both genes are orthologous to tetrapod SOX9 genes. Genetic mapping showed that these two loci reside on chromosome segments that were apparently duplicated in a large-scale genomic duplication event in ray fin fish phylogeny. Both Sox9a and Sox9b proteins bind to the HMG consensus DNA sequences in vitro. We tested different domains for transactivation potential and identified a potential activation domain located in the middle of both Sox9a and Sox9b. During embryogenesis, sox9a and sox9b expression patterns are distinct but overlap in some regions of the brain, head skeleton, and fins. Expression of sox9a/b correlates well with that of col2a1 in chondrogenic elements. In the adults, sox9a is expressed in many tissues including brain, muscle, fin, and testis, whereas sox9b expression is restricted to previtellogenic oocytes of the ovary. This expression pattern predicts that sox9a and sox9b may have unique functions in some specific tissues during development. The role of gene duplication for the evolution of developmental gene function is discussed.",
author = "Chiang, {Evelyn F.L.} and Pai, {Chin I.} and Mary Wyatt and Yan, {Yi Lin} and John Postlethwait and Chung, {Bon Chu}",
year = "2001",
month = "3",
day = "1",
doi = "10.1006/dbio.2000.0129",
language = "English",
volume = "231",
pages = "149--163",
journal = "Developmental Biology",
issn = "0012-1606",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Two sox9 genes on duplicated zebrafish chromosomes

T2 - Expression of similar transcription activators in distinct sites

AU - Chiang, Evelyn F.L.

AU - Pai, Chin I.

AU - Wyatt, Mary

AU - Yan, Yi Lin

AU - Postlethwait, John

AU - Chung, Bon Chu

PY - 2001/3/1

Y1 - 2001/3/1

N2 - Sox9 is a transcription factor required for cartilage formation and testis determination in mammals. We have cloned from zebrafish two sox9 genes, termed sox9a and sox9b. Gene phylogenies showed that both genes are orthologous to tetrapod SOX9 genes. Genetic mapping showed that these two loci reside on chromosome segments that were apparently duplicated in a large-scale genomic duplication event in ray fin fish phylogeny. Both Sox9a and Sox9b proteins bind to the HMG consensus DNA sequences in vitro. We tested different domains for transactivation potential and identified a potential activation domain located in the middle of both Sox9a and Sox9b. During embryogenesis, sox9a and sox9b expression patterns are distinct but overlap in some regions of the brain, head skeleton, and fins. Expression of sox9a/b correlates well with that of col2a1 in chondrogenic elements. In the adults, sox9a is expressed in many tissues including brain, muscle, fin, and testis, whereas sox9b expression is restricted to previtellogenic oocytes of the ovary. This expression pattern predicts that sox9a and sox9b may have unique functions in some specific tissues during development. The role of gene duplication for the evolution of developmental gene function is discussed.

AB - Sox9 is a transcription factor required for cartilage formation and testis determination in mammals. We have cloned from zebrafish two sox9 genes, termed sox9a and sox9b. Gene phylogenies showed that both genes are orthologous to tetrapod SOX9 genes. Genetic mapping showed that these two loci reside on chromosome segments that were apparently duplicated in a large-scale genomic duplication event in ray fin fish phylogeny. Both Sox9a and Sox9b proteins bind to the HMG consensus DNA sequences in vitro. We tested different domains for transactivation potential and identified a potential activation domain located in the middle of both Sox9a and Sox9b. During embryogenesis, sox9a and sox9b expression patterns are distinct but overlap in some regions of the brain, head skeleton, and fins. Expression of sox9a/b correlates well with that of col2a1 in chondrogenic elements. In the adults, sox9a is expressed in many tissues including brain, muscle, fin, and testis, whereas sox9b expression is restricted to previtellogenic oocytes of the ovary. This expression pattern predicts that sox9a and sox9b may have unique functions in some specific tissues during development. The role of gene duplication for the evolution of developmental gene function is discussed.

UR - http://www.scopus.com/inward/record.url?scp=0035281835&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035281835&partnerID=8YFLogxK

U2 - 10.1006/dbio.2000.0129

DO - 10.1006/dbio.2000.0129

M3 - Article

C2 - 11180959

AN - SCOPUS:0035281835

VL - 231

SP - 149

EP - 163

JO - Developmental Biology

JF - Developmental Biology

SN - 0012-1606

IS - 1

ER -