Two-step concerted mechanism for the hydrocarbon hydroxylation by cytochrome P450

Kazunari Yoshizawa, Takehiro Ohta, Masataka Eda, Tokio Yamabe

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

A possible two-step mechanism for the hydrocarbon hydroxylation by the oxoferryl active species of cytochrome P450 is discussed. We propose that the catalytic reaction is initiated by the formation of a reactant complex involving a five-coordinate carbon species or a α-complex, followed by concerted hydrogen and alkyl migrations which lead to the formation of a product complex involving alcohol as a ligand. The porphyrin ring is predicted from molecular mechanics and extended Huckel calculations to be significantly bent in the reactant complex and in the hydroxy intermediate to form an energetically preferred octahedral environment at the iron active center. Such bent structures for the porphyrin ring are consistent with observed cis-coordination geometries for the M(porphyrin)L2 complexes, in which M is a large transition- metal ion and L is a ligand. We suggest that possible inversion at the five-coordinate carbon species in the reactant complex can occur to lead to partial inversion of stereochemistry at a labeled carbon.

Original languageEnglish
Pages (from-to)401-407
Number of pages7
JournalBulletin of the Chemical Society of Japan
Volume73
Issue number2
DOIs
Publication statusPublished - Feb 1 2000
Externally publishedYes

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Hydroxylation
Porphyrins
Hydrocarbons
Cytochrome P-450 Enzyme System
Carbon
Ligands
Stereochemistry
Molecular mechanics
Transition metals
Metal ions
Hydrogen
Iron
Alcohols
Geometry

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

Cite this

Two-step concerted mechanism for the hydrocarbon hydroxylation by cytochrome P450. / Yoshizawa, Kazunari; Ohta, Takehiro; Eda, Masataka; Yamabe, Tokio.

In: Bulletin of the Chemical Society of Japan, Vol. 73, No. 2, 01.02.2000, p. 401-407.

Research output: Contribution to journalArticle

Yoshizawa, Kazunari ; Ohta, Takehiro ; Eda, Masataka ; Yamabe, Tokio. / Two-step concerted mechanism for the hydrocarbon hydroxylation by cytochrome P450. In: Bulletin of the Chemical Society of Japan. 2000 ; Vol. 73, No. 2. pp. 401-407.
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